Simulate_PCR for amplicon prediction and annotation from multiplex, degenerate primers and probes [PDF]
BACKGROUND: Pairing up primers to amplify desired targets and avoid undesired cross reactions can be a combinatorial challenge. Effective prediction of specificity and inclusivity from multiplexed primers and TaqMan®/Luminex® probes is a critical step in
Shea N Gardner, Tom Slezak
core +2 more sources
Advances in Microbial Diagnostics: Machine Learning and Nanotechnology for Zoonotic Disease Control
This review highlights how integrating machine learning with nanotechnology enables enhanced diagnostics, personalized treatments, and the prediction of outbreaks for zoonotic diseases, offering a unified framework to advance global health. ABSTRACT Zoonotic diseases pose significant global health threats, with microbial pathogens, including bacteria ...
Narges Lotfalizadeh +8 more
wiley +1 more source
Protein Nucleotidylylation in +ssRNA Viruses
Nucleotidylylation is a post-transcriptional modification important for replication in the picornavirus supergroup of RNA viruses, including members of the Caliciviridae, Coronaviridae, Picornaviridae and Potyviridae virus families.
Alice-Roza Eruera +3 more
doaj +1 more source
Viable porcine arteriviruses with deletions proximal to the 3 ' end of the genome [PDF]
In order to obtain attenuated live vaccine candidates of porcine reproductive and respiratory syndrome virus (PRRSV), a series of deletions was introduced at the 3′ end of the viral genome using an infectious cDNA clone of the Lelystad virus isolate. RNA
Kroese, M.V. +3 more
core +2 more sources
ABSTRACT Background Respiratory Syncytial Virus (RSV) causes significant respiratory infections, particularly in young children and elderly adults. Genetic variations in the fusion (F) protein can reduce the efficacy of vaccination and monoclonal antibody treatments, emphasizing the need for genomic surveillance of this virus.
Lei Li +6 more
wiley +1 more source
HuCoPIA: An Atlas of Human vs. SARS-CoV-2 Interactome and the Comparative Analysis with Other Coronaviridae Family Viruses [PDF]
Naveen Duhan, Rakesh Kaundal
openalex +1 more source
cis-acting RNA motifs play a major role in regulating many aspects of RNA biology including posttranscriptional processing, nuclear export, RNA localization, translation and degradation.
Gal Haimovich +3 more
semanticscholar +1 more source
Transmissible gastroenteritis virus: Identification of M protein-binding peptide ligands with antiviral and diagnostic potential [PDF]
The membrane (M) protein is one of the major structural proteins of coronavirus particles. In this study, the M protein of transmissible gastroenteritis virus (TGEV) was used to biopan a 12-mer phage display random peptide library.
Ren, Xiaofeng +4 more
core +1 more source
The role of cellular adhesion molecules in virus attachment and entry [PDF]
As obligate intracellular parasites, viruses must traverse the host-cell plasma membrane to initiate infection. This presents a formidable barrier, which they have evolved diverse strategies to overcome.
Bhella, David
core +1 more source
Prevention of Porcine Epidemic Diarrhea Virus With Nanotube‐Adjuvanted Oral DNA Vaccines
Porcine epidemic diarrhea virus (PEDV) causes severe diarrhea in piglets. The ideal route of protection against PEDV for piglets is through passive (lactogenic) immunity, which is not provided by current inactivated and subunit vaccines on the market. In this study, we investigated whether a DNA vaccine encoding the full PEDV spike protein adjuvanted ...
Hsing-Chieh Wu +8 more
wiley +1 more source

