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Correlative Fluorescence and Electron Microscopy [PDF]

open access: yesCurrent Protocols in Cytometry, 2014
AbstractCorrelative fluorescence and electron microscopy (CFEM) is a multimodal technique that combines dynamic and localization information from fluorescence methods with ultrastructural data from electron microscopy, to give new information about how cellular components change relative to the spatiotemporal dynamics within their environment.
Randall T, Schirra, Peijun, Zhang
openaire   +2 more sources

C8‐BTBT‐C8 Thin‐Film Transistors Based on Micro‐Contact Printed PEDOT:PSS/MWCNT Electrodes

open access: yesAdvanced Electronic Materials, 2023
Advances in organic materials manufacturing have enabled the creation of electronic devices using solution‐processing techniques by employing soluble materials with high conductivity grade. In this exploratory study, the use of micro‐contact for poly(3,4‐
Kirill Gubanov   +7 more
doaj   +1 more source

Study of dendritic spine compartmentalization : a correlative fluorescence light microscopy-electron microscopy approach [PDF]

open access: yes, 2014
Neurons communicate with each other through synapses. Most excitatory synapses contact small protrusions called dendritic spines. Spines are connected to dendrites by a very thin stalk called the “spine neck” which restricts diffusion between the spine ...
Cyprien, Vivien
core   +1 more source

Labelling strategies for correlative light electron microscopy

open access: yes, 2023
Imaging is one of the key technologies underpinning discoveries in biomedical research. Each imaging technique however usually only provides a specific type of information.
Tanner, Hugh,   +9 more
core   +1 more source

Correlative Extinction and Single Fluorophore Bleaching Microscopy for Ligand Quantification on Gold Nanoparticles

open access: yesAdvanced Materials Interfaces, 2023
Nanoparticles (NPs) are promising therapeutic delivery agents, with the number and manner of presentation of cell‐binding ligands on the NP affecting the eventual fate of the therapeutic.
Nicole Slesiona   +5 more
doaj   +1 more source

Correlating Fluorescence Microscopy with Electron Microscopy [PDF]

open access: yesMicroscopy Today, 2004
Abstract The use of fluorescent probes is becoming more and more common in cell biology. It would be useful if we were able to correlate a fluorescent structure with an electron microscopic image. The ability to definitively identify a fluorescent organelle would be very valuable.
Stephen W Carmichael, Jon Charlesworth
openaire   +1 more source

Imaging organoids: a correlative light- and electron microscopy approach [PDF]

open access: yes, 2019
Organoids are important pharmacological and disease models. To better study organoids, existing approaches have to be adapted. Correlative microscopy approaches allow for a more holistic understanding of cellular events.
Willemsen, Sam
core   +1 more source

Correlative Organelle Microscopy: Fluorescence Guided Volume Electron Microscopy of Intracellular Processes

open access: yesFrontiers in Cell and Developmental Biology, 2022
Intracellular processes depend on a strict spatial and temporal organization of proteins and organelles. Therefore, directly linking molecular to nanoscale ultrastructural information is crucial in understanding cellular physiology.
Sergey V. Loginov   +9 more
doaj   +1 more source

Correlative photoactivated localization and scanning electron microscopy. [PDF]

open access: yesPLoS ONE, 2013
The ability to localize proteins precisely within subcellular space is crucial to understanding the functioning of biological systems. Recently, we described a protocol that correlates a precise map of fluorescent fusion proteins localized using three ...
Benjamin G Kopek   +4 more
doaj   +1 more source

Correlative Light and Electron Microscopy (CLEM): A Multifaceted Tool for the Study of Geological Specimens

open access: yes, 2023
Correlative light and electron microscopy (CLEM) is an advanced imaging approach that faces critical challenges in the analysis of both materials and biological specimens. CLEM integrates the strengths of both light and electron microscopy, in a hardware
Lucia Miraglia   +9 more
core   +1 more source

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