Guided-deconvolution for correlative light and electron microscopy. [PDF]
PLoS ONE, 2023 Correlative light and electron microscopy is a powerful tool to study the internal structure of cells. It combines the mutual benefit of correlating light (LM) and electron (EM) microscopy information.
Fengjiao Ma +7 more
doaj +3 more sources
Integrated Array Tomography for 3D Correlative Light and Electron Microscopy [PDF]
Frontiers in Molecular Biosciences, 2022 Volume electron microscopy (EM) of biological systems has grown exponentially in recent years due to innovative large-scale imaging approaches. As a standalone imaging method, however, large-scale EM typically has two major limitations: slow rates of ...
Ryan Lane +3 more
doaj +2 more sources
Quantitative 3D correlative light and electron microscopy of organelle association during autophagy [PDF]
Cell Structure and Function, 2022 In macroautophagy, disk-shaped double-membrane structures called phagophores elongate to form cup-shaped structures, becoming autophagosomes upon closure. These autophagosomes then fuse with lysosomes to become autolysosomes and degrade engulfed material.
Satoru Takahashi +3 more
doaj +2 more sources
Photonic-chip assisted correlative light and electron microscopy [PDF]
Communications Biology, 2020 Tinguely et al. develop a photonic chip-based correlative light-electron microscopy system to generate co-registered multi-modal total internal reflection fluorescence microscopy (TIRF) and electron microscopy (EM) images of biological samples at ...
Jean-Claude Tinguely +8 more
doaj +2 more sources
Precise targeting for 3D cryo-correlative light and electron microscopy volume imaging of tissues using a FinderTOP [PDF]
Communications Biology, 2023 Cryo-correlative light and electron microscopy (cryoCLEM) is a powerful strategy to high resolution imaging in the unperturbed hydrated state. In this approach fluorescence microscopy aids localizing the area of interest, and cryogenic focused ion beam ...
Marit de Beer +7 more
doaj +2 more sources
Sorghum bicolor Extracellular Vesicle Isolation, Labeling, and Correlative Light and Electron Microscopy [PDF]
Bio-ProtocolExtracellular vesicles are membrane-bound organelles that play crucial roles in intercellular communication and elicit responses in the recipient cell, such as defense responses against pathogens. In this study, we have optimized a protocol for isolating
Deji Adekanye, Tim Chaya, Jeffrey Caplan
doaj +2 more sources
Simultaneous correlative scanning electron and high-NA fluorescence microscopy. [PDF]
PLoS ONE, 2013 Correlative light and electron microscopy (CLEM) is a unique method for investigating biological structure-function relations. With CLEM protein distributions visualized in fluorescence can be mapped onto the cellular ultrastructure measured with ...
Nalan Liv +9 more
doaj +2 more sources
Imaging VIPER-labeled Cellular Proteins by Correlative Light and Electron Microscopy [PDF]
Bio-Protocol, 2019 Advances in fluorescence microscopy (FM), electron microscopy (EM), and correlative light and EM (CLEM) offer unprecedented opportunities for studying diverse proteins and nanostructures involved in fundamental cell biology.
Julia Doh +4 more
doaj +2 more sources
Bimodal endocytic probe for three-dimensional correlative light and electron microscopy. [PDF]
Cell Rep Methods, 2022 Fermie J +17 more
europepmc +2 more sources
Correlative Light and Electron Microscopy Using Frozen Section Obtained Using Cryo-Ultramicrotomy. [PDF]
Int J Mol Sci, 2021 Kim HL, Riew TR, Park J, Lee Y, Kim IB.
europepmc +2 more sources