Results 21 to 30 of about 12,575 (237)

A Brief History of Microglial Ultrastructure: Distinctive Features, Phenotypes, and Functions Discovered Over the Past 60 Years by Electron Microscopy

Frontiers in Immunology, 2018
The first electron microscope was constructed in 1931. Several decades later, techniques were developed to allow the first ultrastructural analysis of microglia by transmission electron microscopy (EM).
Julie C. Savage, Julie C. Savage, Katherine Picard, Katherine Picard, Fernando González-Ibáñez, Fernando González-Ibáñez, Marie-Ève Tremblay, Marie-Ève Tremblay   +7 more
doaj   +1 more source

Correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in Mycobacterium tuberculosis-infected lungs.

PLoS Biology, 2020
Correlative light, electron, and ion microscopy (CLEIM) offers huge potential to track the intracellular fate of antibiotics, with organelle-level resolution.
Antony Fearns, Daniel J Greenwood, Angela Rodgers, Haibo Jiang, Maximiliano G Gutierrez   +4 more
doaj   +1 more source

Fluorescent and Electron-Dense Green Color Emitting Nanodiamonds for Single-Cell Correlative Microscopy

Molecules, 2020
Correlative light and electron microscopy (CLEM) is revolutionizing how cell samples are studied. CLEM provides a combination of the molecular and ultrastructural information about a cell.
Neeraj Prabhakar, Markus Peurla, Olga Shenderova, Jessica M. Rosenholm   +3 more
doaj   +1 more source

Optimization of negative stage bias potential for faster imaging in large-scale electron microscopy

Journal of Structural Biology: X, 2021
Large-scale electron microscopy (EM) allows analysis of both tissues and macromolecules in a semi-automated manner, but acquisition rate forms a bottleneck.
Ryan Lane, Yoram Vos, Anouk H.G. Wolters, Luc van Kessel, S. Elisa Chen, Nalan Liv, Judith Klumperman, Ben N.G. Giepmans, Jacob P. Hoogenboom   +8 more
doaj   +1 more source

Customized patterned substrates for highly versatile correlative light-scanning electron microscopy [PDF]

, 2014
Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each
S. Rodighiero, P. Milani, L. Benedetti, E. Sogne, M. Francolini, D. Marchesi, Rodighiero Simona, Francolini Maura, Sogne Elisa, Marchesi Davide, Milani Paolo, Benedetti Lorena   +11 more
core   +1 more source

Imaging organoids: a correlative light- and electron microscopy approach [PDF]

, 2019
Organoids are important pharmacological and disease models. To better study organoids, existing approaches have to be adapted. Correlative microscopy approaches allow for a more holistic understanding of cellular events.
Willemsen, Sam
core   +1 more source

Improved volume CLEM revealed that aberrant phagophores and RB1CC1/FIP200-containing clusters appear surround SQSTM1/p62 aggregates in Atg9a-deficient cells

Autophagy Reports, 2023
ATG9A is an important membrane protein in mammalian macroautophagy. The formation of autophagosomes and phagophores is blocked in atg9a KO cells. However, it remains possible that residual membrane formation activity exists in these cells.
Soichiro Kakuta, Junji Yamaguchi, Chigure Suzuki, Isei Tanida, Yasuo Uchiyama   +4 more
doaj   +1 more source

Imaging Viral Factories

Proceedings, 2020
Viruses remodel cellular compartments to build their replication factories. Remarkably, viruses are also able to induce new membranes and new organelles.
Cristina Risco Ortiz
doaj   +1 more source

MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy

eLife, 2019
The non-destructive collection of ultrathin sections on silicon wafers for post-embedding staining and volumetric correlative light and electron microscopy traditionally requires exquisite manual skills and is tedious and unreliable.
Thomas Templier
doaj   +1 more source

Light microscopy of proteins in their ultrastructural context

Nature Communications, 2020
Imaging specific proteins in the ultrastructural context largely relies on correlative light/electron microscopy, but fluorophore incompatibility and registration issues limit its use.
Ons M’Saad, Joerg Bewersdorf
doaj   +1 more source