Results 1 to 10 of about 16,598 (199)

A novel method of correlative light and electron microscopy in cryosectioning of bovine anterior pituitary tissue using NanoSuit CLEM [PDF]

open access: yesThe Journal of Reproduction and Development
Correlative light-electron microscopy (CLEM) combines fluorescence microscopy and scanning electron microscopy (SEM) to achieve nanoscale resolution while highlighting regions of interest identified by fluorescence microscopy.
Hiroya KADOKAWA, Hideya KAWASAKI
doaj   +2 more sources

Rapid in-EPON CLEM: Combining fast and efficient labeling of self-labeling enzyme tags with EM-resistant Janelia Fluor dyes and StayGold

open access: yesHeliyon
Correlative light and electron microscopy (CLEM) combines light microscopy (LM) of fluorescent samples to ultrastructural analyses by electron microscopy (EM).
Michael Hensel   +2 more
exaly   +3 more sources

A Step-By-Step Protocol for Correlative Light and Electron Microscopy Imaging of Proteinaceous Deposits in Cultured Cells and Human Brain Tissues [PDF]

open access: yesBio-Protocol
An improved correlative light and electron microscopy (CLEM) method has recently been introduced and successfully employed to identify and analyze protein inclusions in cultured cells as well as pathological proteinaceous deposits in postmortem human ...
Peizhou Jiang, Dennis Dickson
doaj   +2 more sources

Application of immuno- and affinity labeling with fluorescent dyes to in-resin CLEM of Epon-embedded cells

open access: yesHeliyon, 2023
In-resin CLEM (Correlative Light and Electron Microscopy) of Epon-embedded cells involves correlating fluorescence microscopy with electron microscopy in the same Epon-embedded ultrathin section.
Isei Tanida   +4 more
doaj   +1 more source

Efficient differentiation of human embryonic stem cells to retinal pigment epithelium under defined conditions

open access: yesStem Cell Research & Therapy, 2021
Age-related macular degeneration (AMD) is a highly prevalent form of blindness caused by loss death of cells of the retinal pigment epithelium (RPE). Transplantation of pluripotent stem cell (PSC)-derived RPE cells is considered a promising therapy to ...
Ioannis J. Limnios   +4 more
doaj   +1 more source

Recent technological advances in correlative light and electron microscopy for the comprehensive analysis of neural circuits

open access: yesFrontiers in Neuroanatomy, 2022
Light microscopy (LM) covers a relatively wide area and is suitable for observing the entire neuronal network. However, resolution of LM is insufficient to identify synapses and determine whether neighboring neurons are connected via synapses.
Hirohide Iwasaki   +3 more
doaj   +1 more source

In vitro modulation of Schwann cell behavior by VEGF and PDGF in an inflammatory environment

open access: yesScientific Reports, 2022
Peripheral glial cell transplantation with Schwann cells (SCs) is a promising approach for treating spinal cord injury (SCI). However, improvements are needed and one avenue to enhance regenerative functional outcomes is to combine growth factors with ...
Souptik Basu   +7 more
doaj   +1 more source

Two-color in-resin CLEM of Epon-embedded cells using osmium resistant green and red fluorescent proteins

open access: yesScientific Reports, 2020
In-resin CLEM of Epon embedded samples can greatly simplify the correlation of fluorescent images with electron micrographs. The usefulness of this technique is limited at present by the low number of fluorescent proteins that resist CLEM processing ...
Isei Tanida   +5 more
doaj   +1 more source

Examining Nanoparticle Interactions on a Cellular Scale Using Correlative Light Electron Microscopy

open access: yesMolecular Biology & Nanomedicine, 2021
One key area of nanomedicine research isthe mechanism of action of nanoparticles within the body, it’s biodistributionand pharmacokinetic properties. However, insight into the nanoparticle-cellinterface is limited, due in part to the heterogeneous nature
Brian Caffrey
doaj   +1 more source

PREPARATION TO COLONOSCOPY COMPLEMENTED BY MODERN ADJUSTING METHODS IN PATIENTS WITH EPITHELIAL LESIONS OF THE COLON

open access: yesМедицинский совет, 2017
The article provides analysis of the study of diagnostic value of the epithelial lesions of the column – a combination of newest visualization methods as NBI and ZOOM with confocal laser endomicroscopy. The study was conducted in 202 patients aged 40–84,
A. G. Shuleshova   +4 more
doaj   +1 more source

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