Results 1 to 10 of about 14,888,742 (175)

FlyTomo: a streamlined software for on-the-fly cryo-ET data processing and diagnosis [PDF]

Nature Communications
Cryo-ET combined with subtomogram averaging (STA) enables the structural elucidation of macromolecular assemblies in native environments. However, their widespread adoption has been limited by the labor-intensive, expertise-dependent data processing ...
Zheyuan Zhang, Cheng Peng, Weiping Zhang, Jiaming Liang, Kexin Liu, Yong Chen, Junxia Zhang, Rui Liang, Yutong Song, Sai Li   +9 more
doaj   +2 more sources

RETRACTED ARTICLE: A hybrid YOLO-UNet3D framework for automated protein particle annotation in Cryo-ET images

Scientific Reports
Accurate localization and identification of protein complexes in cryo-electron tomography (cryo-ET) volumes are essential for understanding cellular functions and disease mechanisms.
Ziyang Liu, Chunhong Yuan, Zixin Zhang, Xiang Zhou, Xiangyu Li, Zhen Tian, Yuting Pei, Zuowen Jiang, Zhikui Tian   +8 more
doaj   +2 more sources

Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ

eLife, 2023
Nuclear processes depend on the organization of chromatin, whose basic units are cylinder-shaped complexes called nucleosomes. A subset of mammalian nucleosomes in situ (inside cells) resembles the canonical structure determined in vitro 25 years ago ...
Zhi Yang Tan, Shujun Cai, Alex J Noble, Jon K Chen, Jian Shi, Lu Gan   +5 more
doaj   +1 more source

Self-supervised learning for macromolecular structure classification based on cryo-electron tomograms

Frontiers in Physiology, 2022
Macromolecular structure classification from cryo-electron tomography (cryo-ET) data is important for understanding macro-molecular dynamics. It has a wide range of applications and is essential in enhancing our knowledge of the sub-cellular environment.
Tarun Gupta, Xuehai He, Mostofa Rafid Uddin, Xiangrui Zeng, Andrew Zhou, Jing Zhang, Zachary Freyberg, Min Xu   +7 more
doaj   +1 more source

Focused Ion Beam Milling and Cryo-electron Tomography Methods to Study the Structure of the Primary Cell Wall in Allium cepa

Bio-Protocol, 2022
Cryo-electron tomography (cryo-ET) is a formidable technique to observe the inner workings of vitrified cells at a nanometric resolution in near-native conditions and in three-dimensions. One consequent drawback of this technique is the sample thickness,
William Nicolas, Grant Jensen, Elliot Meyerowitz   +2 more
doaj   +1 more source

Computational methods for in situ structural studies with cryogenic electron tomography

Frontiers in Cellular and Infection Microbiology, 2023
Cryo-electron tomography (cryo-ET) plays a critical role in imaging microorganisms in situ in terms of further analyzing the working mechanisms of viruses and drug exploitation, among others.
Cuicui Zhao, Da Lu, Qian Zhao, Chongjiao Ren, Huangtao Zhang, Jiaqi Zhai, Jiaxin Gou, Shilin Zhu, Yaqi Zhang, Xinqi Gong, Xinqi Gong   +10 more
doaj   +1 more source

Preparing Arabidopsis thaliana root protoplasts for cryo electron tomography

Frontiers in Plant Science, 2023
The use of protoplasts in plant biology has become a convenient tool for the application of transient gene expression. This model system has allowed the study of plant responses to biotic and abiotic stresses, protein location and trafficking, cell wall ...
Ingrid Berenice Sanchez Carrillo, Patrick C. Hoffmann, Teura Barff, Martin Beck, Martin Beck, Hugo Germain   +5 more
doaj   +1 more source

Mind the Gap: How Some Viruses Infect Their Hosts

Viruses, 2010
Cryo-electron microscopy (Cryo-EM) and cryo-electron tomography (Cryo-ET) provide structural insights into complex biological processes. The podoviridae are dsDNA containing phage with short, non-contractile tails which nevertheless translocate their DNA
Peter E. Prevelige
doaj   +1 more source

MDTOMO method for continuous conformational variability analysis in cryo electron subtomograms based on molecular dynamics simulations

Scientific Reports, 2023
Cryo electron tomography (cryo-ET) allows observing macromolecular complexes in their native environment. The common routine of subtomogram averaging (STA) allows obtaining the three-dimensional (3D) structure of abundant macromolecular complexes, and ...
Rémi Vuillemot, Isabelle Rouiller, Slavica Jonić   +2 more
doaj   +1 more source

Skeletal muscle triad junction ultrastructure by Focused-Ion-Beam milling of muscle and Cryo-Electron Tomography

European Journal of Translational Myology, 2015
Cryo-electron tomography (cryo-ET) has emerged as perhaps the only practical technique for revealing nanometer-level three-dimensional structural details of subcellular macromolecular complexes in their native context, inside the cell.
Terence Wagenknecht, Chyongere Hsieh, Michael Marko   +2 more
doaj   +1 more source