Results 1 to 10 of about 2,441,948 (283)
Cryo-electron tomography pipeline for plasma membranes [PDF]
Nature CommunicationsCryo-electron tomography (cryoET) provides sub-nanometer protein structure within the dense cellular environment. Existing sample preparation methods are insufficient at accessing the plasma membrane and its associated proteins.
Willy W. Sun +8 more
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Quantitative Cryo-Electron Tomography
Frontiers in Molecular Biosciences, 2022 The three-dimensional organization of biomolecules important for the functioning of all living systems can be determined by cryo-electron tomography imaging under native biological contexts. Cryo-electron tomography is continually expanding and evolving,
Paula P. Navarro, Paula P. Navarro
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Tracing low-level structures in cryo-electron tomography. [PDF]
PLoS ONECryo-electron tomography is an imaging technique that provides 3D images (tomograms) in situ of cells with sub-nanometer resolution. Typically, the first step in the analysis is to classify the tomogram voxels into different structures, named semantic ...
Pelayo Alvarez Brecht +5 more
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Recent Advances in Cryo Electron Microscopy and Tomography [PDF]
Structural DynamicsIn the recent years, cryo-electron microscopy (cryo-EM) single-particle analysis (SPA) has greatly benefited from a multitude of technological advances cumulating in breaking the atomic-resolution barrier for biological macromolecules.
Eliza Nieweglowska +2 more
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Preparing Arabidopsis thaliana root protoplasts for cryo electron tomography [PDF]
Frontiers in Plant Science, 2023 The use of protoplasts in plant biology has become a convenient tool for the application of transient gene expression. This model system has allowed the study of plant responses to biotic and abiotic stresses, protein location and trafficking, cell wall ...
Ingrid Berenice Sanchez Carrillo +5 more
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Labeling and Localization Strategies for In Situ Cryo-Electron Tomography Across the Viral Life Cycle [PDF]
VirusesCryo-electron tomography (Cryo-ET) has emerged as a transformative tool for visualizing viral components within their native cellular environment, enabling structural interrogation of viral life cycle events at nanometer resolution without chemical ...
Yoon Ho Park +3 more
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Visualization of lysosomal membrane proteins by cryo electron tomography [PDF]
Nature CommunicationsLysosomes are essential organelles for cellular homeostasis and signaling, with dysfunction linked to neurological disorders, lysosomal storage diseases, and cancer. While proteomics has advanced our understanding of lysosomal composition, the structural
Bridget M. McVeigh +7 more
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Progress in the development of an ultrafast pulsed ponderomotive phase plate for cryo-electron tomography [PDF]
Structural DynamicsCryo-electron tomography (cryo-ET) is a powerful modality for resolving cellular structures in their native state. While single-particle cryo-electron microscopy excels in determining protein structures purified from recombinant or endogenous sources due
Daniel X. Du +9 more
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Cryo electron tomography of native HIV-1 budding sites. [PDF]
PLoS Pathogens, 2010 The structure of immature and mature HIV-1 particles has been analyzed in detail by cryo electron microscopy, while no such studies have been reported for cellular HIV-1 budding sites.
Lars-Anders Carlson +6 more
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Journal of Structural Biology: X, 2022 Advancements in the field of cryo-electron tomography have greatly contributed to our current understanding of prokaryotic cell organization and revealed intracellular structures with remarkable architecture.
Janine Liedtke +2 more
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