Results 11 to 20 of about 2,441,948 (283)

Cryo-electron tomography of biological specimens [PDF]

open access: yesIEEE Signal Processing Magazine, 2006
Cryo-electron tomography (CET) is an imaging technique capable of visualizing the three-dimensional (3-D) structure of complex viruses, cells, and tissues in hydrated state.
Leis, A.   +13 more
core   +7 more sources

Challenges and triumphs in cryo-electron tomography [PDF]

open access: yesiScience, 2021
Summary: Cryo-electron tomography has stepped fully into the spotlight. Enthusiasm is high. Fortunately for us, this is an exciting time to be a cryotomographer, but there is still a way to go before declaring victory.
Ryan K. Hylton, Matthew T. Swulius
doaj   +3 more sources

Cryo-electron tomography

open access: yes, 2023
Electron tomography in biology is a technique to reconstruct the three-dimensional structure of macro-molecular, sub-cellular or cellular objects by transmission electron microscopy (TEM).
Ishikawa, Takashi (author)
core   +2 more sources

Neurons as a model system for cryo-electron tomography. [PDF]

open access: yesJournal of Structural Biology: X, 2022
Cryo-electron tomography (Cryo-ET) provides unique opportunities to image cellular components at high resolution in their native state and environment.
Lučić, Vladan   +5 more
core   +6 more sources

Cryo-Electron Tomography of the Mammalian Synapse [PDF]

open access: yes, 2018
Characterizing the detailed structure of the mammalian synapse is of crucial importance to understand its mechanisms of function. Here I describe a protocol to study synaptic architecture by cryo-electron tomography (cryo-ET), a powerful electron ...
Rubén Fernández-Busnadiego   +1 more
core   +5 more sources

Identifying the assembly pathway of cyanophage inside the marine bacterium using electron cryo-tomography

open access: yesMicrobial Cell, 2014
Advances in electron cryo-tomography open up a new avenue to visualize the 3-D internal structure of a single bacterium before and after its infection by bacteriophages in its native environment, without using chemical fixatives, fluorescent dyes or ...
Wei Dai   +3 more
doaj   +2 more sources

Cryo-Electron Tomography of Reconstituted Biomolecular Condensates

open access: yes, 2023
The assembly of membraneless compartments by phase separation has recently been recognized as a mechanism for spatial and temporal organization of biomolecules within the cell.
Woodruff, J.   +6 more
core   +4 more sources

HOPE-SIM, a cryo-structured illumination fluorescence microscopy system for accurately targeted cryo-electron tomography

open access: yesCommunications Biology, 2023
Cryo-focused ion beam (cryo-FIB) milling technology has been developed for the fabrication of cryo-lamella of frozen native specimens for study by in situ cryo-electron tomography (cryo-ET).
Shuoguo Li   +8 more
doaj   +2 more sources

Cryo-Electron Tomography and Subtomogram Averaging

open access: yes, 2016
Cryo-electron tomography (cryo-ET) allows 3D volumes to be reconstructed from a set of 2D projection images of a tilted biological sample. It allows densities to be resolved in 3D that would otherwise overlap in 2D projection images.
Briggs, J.   +3 more
core   +4 more sources

LIMITING FACTORS IN SINGLE PARTICLE CRYO ELECTRON TOMOGRAPHY

open access: yesComputational and Structural Biotechnology Journal, 2012
Modern methods of cryo electron microscopy and tomography allow visualization of protein nanomachines in their native state at the nanometer scale. Image processing methods including sub-volume averaging applied to repeating macromolecular elements ...
Mikhail Kudryashev   +2 more
doaj   +3 more sources

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