Results 1 to 10 of about 56,400 (87)

HOPE-SIM, a cryo-structured illumination fluorescence microscopy system for accurately targeted cryo-electron tomography

Communications Biology, 2023
Cryo-focused ion beam (cryo-FIB) milling technology has been developed for the fabrication of cryo-lamella of frozen native specimens for study by in situ cryo-electron tomography (cryo-ET).
Shuoguo Li, Xing Jia, Tongxin Niu, Xiaoyun Zhang, Chen Qi, Wei Xu, Hongyu Deng, Fei Sun, Gang Ji   +8 more
doaj   +2 more sources

A modular platform for automated cryo-FIB workflows

eLife, 2021
Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB) has matured into a preparation method widely used for cellular cryo-electron tomography.
Sven Klumpe, Herman KH Fung, Sara K Goetz, Ievgeniia Zagoriy, Bernhard Hampoelz, Xiaojie Zhang, Philipp S Erdmann, Janina Baumbach, Christoph W Müller, Martin Beck, Jürgen M Plitzko, Julia Mahamid   +11 more
doaj   +2 more sources

Super-resolution confocal cryo-CLEM with cryo-FIB milling for in situ imaging of Deinococcus radiodurans

Current Research in Structural Biology, 2022
Studying bacterial cell envelope architecture with electron microscopy is challenging due to the poor preservation of microbial ultrastructure with traditional methods.
Danielle L. Sexton, Steffen Burgold, Andreas Schertel, Elitza I. Tocheva   +3 more
doaj   +2 more sources

Correlative Light and Electron Cryo-Microscopy Workflow Combining Micropatterning, Ice Shield, and an In-Chamber Fluorescence Light Microscope

Bio-Protocol, 2023
In situ cryo-electron tomography (cryo-ET) is the most current, state-of-the-art technique to study cell machinery in its hydrated near-native state. The method provides ultrastructural details at sub-nanometer resolution for many components within the ...
Sabrina Berkamp, Deniz Daviran, Marit Smeets, Alexane Caignard, Riddhi Jani, Pia Sundermeyer, Caspar Jonker, Sven Gerlach, Bernd Hoffmann, Katherine Lau, Carsten Sachse   +10 more
doaj   +2 more sources

How advances in cryo-electron tomography have contributed to our current view of bacterial cell biology

Journal of Structural Biology: X, 2022
Advancements in the field of cryo-electron tomography have greatly contributed to our current understanding of prokaryotic cell organization and revealed intracellular structures with remarkable architecture.
Janine Liedtke, Jamie S. Depelteau, Ariane Briegel   +2 more
doaj   +3 more sources

Cryo-CLXEM introduces cryo-SXT to bridge the resolution gap in cryo-CLEM

Communications Biology
Cryo-imaging in cellular biology provides the means to visualize the cellular interior at close-to-native conditions. A cornerstone in the field has been cryo-correlative light and electron microscopy (cryo-CLEM), with cryo-visible light fluorescent ...
Johannes Groen, Anastasia Gazi, Sergey Kapishnikov, Anne Brelot, Matthijn Vos, Jost Enninga, Eva Pereiro, Anna Sartori-Rupp   +7 more
doaj   +2 more sources

Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies

Communications Biology, 2021
Using the post-correlation on-lamella cryo-CLEM workflow, Klein, Wimmer et al. show that lamellar bodies (LBs) are composed of membrane sheets frequently attached to the limiting membrane through T-junctions in ABCA3 overexpressing cells and in primary ...
Steffen Klein, Benedikt H. Wimmer, Sophie L. Winter, Androniki Kolovou, Vibor Laketa, Petr Chlanda   +5 more
doaj   +1 more source

Electron cryo-tomography reveals the subcellular architecture of growing axons in human brain organoids

eLife, 2021
During brain development, axons must extend over great distances in a relatively short amount of time. How the subcellular architecture of the growing axon sustains the requirements for such rapid build-up of cellular constituents has remained elusive ...
Patrick C Hoffmann, Stefano L Giandomenico, Iva Ganeva, Michael R Wozny, Magdalena Sutcliffe, Madeline A Lancaster, Wanda Kukulski   +6 more
doaj   +1 more source

Serial-section Electron Tomography and Quantitative Analysis of Microtubule Organization in 3D-reconstructed Mitotic Spindles

Bio-Protocol, 2023
For the analysis of cellular architecture during mitosis, nanometer resolution is needed to visualize the organization of microtubules in spindles. Here, we present a detailed protocol that can be used to produce 3D reconstructions of whole mitotic ...
Robert Kiewisz, Daniel Baum, Thomas Müller-Reichert, Gunar Fabig   +3 more
doaj   +1 more source

How Membrane Contact Sites Shape the Phagophore

Contact, 2023
During macroautophagy, phagophores establish multiple membrane contact sites (MCSs) with other organelles that are pivotal for proper phagophore assembly and growth. In S.
Cristina Capitanio, Anna Bieber, Florian Wilfling   +2 more
doaj   +1 more source