Results 11 to 20 of about 56,400 (87)

INS-1E cryo-CLEM calreticulin

, 2015
Raw data files of INS-1E cryo-CLEM calreticulin<strong>Tilt Series Date:</strong> 2015-05-21</p> <strong>Data Taken By:</strong> Stephen Carter</p> <strong>Species / Specimen:</strong> INS-1E Pancreatic ...
Carter, Stephen
core   +14 more sources

Adapting cryogenic correlative light and electron microscopy (cryo-CLEM) for food oxidation studies [PDF]

, 2023
Lipid oxidation is a major cause of product deterioration in protein stabilised oil-in-water food emulsions. The impact of protein emulsifiers on lipid oxidation and the stability depends on the specific type of protein emulsifiers used and the redox ...
Johannes, Hohlbein, Suyeon, Yang, John, van Duynhoven, Heiner, Friedrich, Rick, Joosten, Machi, Takeuchi   +5 more
core   +1 more source

cryo-CLEM LC3

, 2020
Raw data files of cryo-CLEM LC3<strong>Tilt Series Date:</strong> 2020-02-09</p> <strong>Data Taken By:</strong> Stephen Carter</p> <strong>Species / Specimen:</strong> Cell line 25</p> <strong> ...
Carter, Stephen
core   +5 more sources

Untangling the Microscopic World of Organelles, Cells, Tissues, and Organs: A Focus on the Dysfunctional Golgi Apparatus in Disease Research

Biology and Life Sciences Forum, 2023
Emerging techniques in organelle structural biology have revolutionized our understanding of disease mechanisms and opened new possibilities for developing targeted therapies.
Daniel Joseph Gómez
doaj   +1 more source

Advanced cryo-tomography workflow developments - correlative microscopy, milling automation and cryo-lift-out [PDF]

, 2020
Cryo-electron tomography (cryo-ET) is a groundbreaking technology for 3D visualisation and analysis of biomolecules in the context of cellular structures. It allows structural investigations of single proteins as well as their spatial arrangements within
Erdmann, P., Goetze, B., Kuba, J., Rigort, A., Berka, L., Kirmse, R., Mitchels, J., De Bock, J., Hovorka, M., König, J.   +9 more
core   +1 more source

Expanding the Toolkit for Structural Cell Biology with ExoSloNano [PDF]

Structural Dynamics
In situ cryo-Electron Microscopy (cryo-EM) enables the direct interrogation of structure-function relationships by resolving macromolecular structures in their native cellular environment.
Lindsey N Young, Alice Sherrard, Huabin Zhou, Farhaz Shaikh, Joshua Hutchings, Margo Riggi, Mythreyi Narasimhan, Eric Bennett, Michael Rosen, Antonio Giraldez, Elizabeth Villa   +10 more
doaj   +1 more source

Micropatterning of cells on EM grids for efficient cryo-correlative light electron microscopy

, 2021
International audienceCryo-transmission electron microscopy (cryo-TEM) provides access to high resolution information of adherent cell ultrastructures in a close to native environment but only volumes of less than 500 nm can be imaged.
Léa Swistak, Sartori-Rupp, Anna, Jost Enninga, Anna Sartori-Rupp, Vos, Matthijn, Matthijn Vos, Swistak, Léa, Enninga, Jost   +7 more
core   +1 more source

In situ microfluidic cryofixation for cryo Focused Ion Beam milling and cryo electron tomography. [PDF]

, 2019
We present a microfluidic platform for studying structure-function relationships at the cellular level by connecting video rate live cell imaging with in situ microfluidic cryofixation and cryo-electron tomography of near natively preserved, unstained ...
Messling, J., Plitzko, J., Fuest, M., Galilea-Kleinsteuber, Rodrigo I., Nocera, G., Galilea-Kleinsteuber, R., Messling, Jan-Erik, Heymann, Michael, Burg, Thomas P., Plitzko, Jürgen M., Nocera, Giovanni Marco, Burg, Thomas Peter, Burg, T., Schaffer, Miroslava, Heymann, M., Galilea-Kleinsteuber, Rodrigo I, Schaffer, M., Fuest, Marie   +17 more
core   +1 more source

PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy

eLife, 2019
Cryo-electron tomography (cryo-ET) is emerging as a revolutionary method for resolving the structure of macromolecular complexes in situ. However, sample preparation for in situ Cryo-ET is labour-intensive and can require both cryo-lamella preparation ...
Sergey Gorelick, Genevieve Buckley, Gediminas Gervinskas, Travis K Johnson, Ava Handley, Monica Pia Caggiano, James C Whisstock, Roger Pocock, Alex de Marco   +8 more
doaj   +1 more source

Molecular and topological reorganizations in mitochondrial architecture interplay during Bax-mediated steps of apoptosis

eLife, 2019
During apoptosis, Bcl-2 proteins such as Bax and Bak mediate the release of pro-apoptotic proteins from the mitochondria by clustering on the outer mitochondrial membrane and thereby permeabilizing it.
Nicholas R Ader, Patrick C Hoffmann, Iva Ganeva, Alicia C Borgeaud, Chunxin Wang, Richard J Youle, Wanda Kukulski   +6 more
doaj   +1 more source