A modular platform for automated cryo-FIB workflows [PDF]
eLife, 2021 Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB) has matured into a preparation method widely used for cellular cryo-electron tomography.
Sven Klumpe +11 more
doaj +8 more sources
Sample preparation and image registration for correlative cryo-FM and cryo-FIB-SEM of plunge-frozen mammalian cells. [PDF]
STAR Protocols, 2022 Summary: We recently demonstrated how lipid droplets can serve as in situ fiducials for correlating cryo-fluorescence microscopy (cryo-FM) and cryo-focused ion beam scanning electron microscopy (cryo-FIB-SEM) datasets of mammalian cells grown on grids ...
Perrine Paul-Gilloteaux +2 more
exaly +6 more sources
Quantification of gallium cryo-FIB milling damage in biological lamellae [PDF]
Proc Natl Acad Sci U S A, 2023 Cryogenic electron microscopy (cryo-EM) has the potential to reveal the molecular details of biological processes in their native, cellular environment at atomic resolution.
Bronwyn A. Lucas, N. Grigorieff
semanticscholar +7 more sources
In situ cryo-FIB/SEM Specimen Preparation Using the Waffle Method
Bio-Protocol, 2022 Cryo-focused ion beam (FIB) milling of vitrified specimens is emerging as a powerful method for in situ specimen preparation. It allows for the preservation of native and near-native conditions in cells, and can reveal the molecular structure of protein ...
Oleg Klykov +7 more
doaj +5 more sources
Super-resolution confocal cryo-CLEM with cryo-FIB milling for in situ imaging of Deinococcus radiodurans [PDF]
Current Research in Structural Biology, 2022 Studying bacterial cell envelope architecture with electron microscopy is challenging due to the poor preservation of microbial ultrastructure with traditional methods.
Danielle L. Sexton +3 more
doaj +4 more sources
Cryo-FIB–enabled 3D imaging of biological specimens [PDF]
Applied MicroscopyCryo-electron tomography (cryo-ET) has become a key technique for observing the three-dimensional structures of biomolecular complexes and organelles in cellular and tissue environments in situ.
Seonhye Son +3 more
doaj +4 more sources
In-cell chromatin structure by Cryo-FIB and Cryo-ET [PDF]
Curr Opin Struct BiolChromatin, the complex of DNA and proteins that organises genetic material in eukaryotic cells, has been a focal point of biological research for over a century.
Zhen Hou, Pei-Jun Zhang
semanticscholar +4 more sources
Logistics of Bone Mineralization in the Chick Embryo Studied by 3D Cryo FIB‐SEM Imaging [PDF]
Advanced Science, 2023 During skeletal development, bone growth and mineralization require transport of substantial amounts of calcium, while maintaining very low concentration. How an organism overcomes this major logistical challenge remains mostly unexplained.
Emeline Raguin +4 more
doaj +3 more sources
Reproducible Sample Preparation of Virus-Infected Cells for Cryo-FIB/ET Using Manual Plunge Freezing [PDF]
Bio-ProtocolMost viruses extensively remodel their host cells to establish productive infection. Visualization of virus-induced cellular remodeling by electron microscopy (EM) has been revolutionized in recent years by advances in cryo-focused ion beam (cryo-FIB ...
Nathalie Lavoie, Ekaterina Heldwein
doaj +3 more sources
Cryo-FIB specimen preparation for use in a cartridge-type cryo-TEM [PDF]
Journal of Structural Biology, 2017 Cryo-electron tomography (cryo-ET) is a well-established technique for studying 3D structural details of subcellular macromolecular complexes and organelles in their nearly native context in the cell. A primary limitation of the application of cryo-ET is the accessible specimen thickness, which is less than the diameters of almost all eukaryotic cells.
Haixin Sui
exaly +5 more sources