Results 41 to 50 of about 177,867 (249)

Recent Advances in Gas Injection System-Free Cryo-FIB Lift-Out Transfer for Cryo-Electron Tomography of Multicellular Organisms and Tissues

Microscopy Today, 2022
Cryo-electron tomography (cryo-ET) enables visualization of protein complexes within their native cellular environment at molecular resolution. Most cells and all tissues, however, are too thick to be imaged directly by transmission electron microscopy ...
Sven Klumpe, J. Kuba, Oda H. Schioetz, P. Erdmann, A. Rigort, J. Plitzko   +5 more
semanticscholar   +1 more source

Direct Measurement of Mechanical Properties of Vitreous Ice by Cryo-FIB.

Microscopy and Microanalysis, 2023
Heonhwa Choi, E. Firlar, J. Pénzes, A. Mann, J. Kaelber   +4 more
semanticscholar   +2 more sources

Cryo-FIB Preparation for Cryo-TEM Tomography [PDF]

Microscopy and Microanalysis, 2010
Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – August 5, 2010.
M Marko, C Hsieh, D Vetter, NJ Salmon, C Mannella   +4 more
openaire   +1 more source

Cryo-focus ion beam (Cryo-FIB) and cryo-electron tomography (Cryo-ET) analyses of APCs in vps21Δ cells under conditions representative of nitrogen starvation.

, 2022
Cells of vps21Δ were grown and treated as described in Fig 3A. After subjecting the cells to nitrogen starvation for 2 or 8 h, they were immediately analyzed using the Cryo-FIB and Cryo-ET methods, as described in the Materials and methods section.
Haiqian Xu (13955194), Yun Chen (279569), Xia You (11934092), Fan Zhou (196667), Xiaomin Zhao (566041), Xiangrui Guo (460265), Rui Li (4631), Zulin Wu (4451572), Ling Liu (143030), Juan Cai (470988), Zhiping Xie (743301), Chuanhai Fu (19285), Yanhong Xue (4162945), Junze Liu (4225276), Tingting Xie (837869), Jiajie Feng (1324467), Pei Wang (102036), Yongheng Liang (4451560)   +17 more
core   +1 more source

Cryo-FIB for TEM investigation of soft matter and beam sensitive energy materials

Nanotechnology, 2022
Primarily driven by structural biology, the rapid advances in cryogenic electron microscopy techniques are now being adopted and applied by materials scientists.
Daniel M Long, Manish Singh, Kathryn A. Small, J. Watt   +3 more
semanticscholar   +1 more source

A Modular Platform for Streamlining Automated Cryo-FIB Workflows [PDF]

, 2021
Abstract Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB) has matured into a preparation method widely used for cellular cryo-electron tomography. Due to the limited ablation rates of low Ga + ion beam currents required to maintain the ...
Sven Klumpe, Herman K. H. Fung, Sara K. Goetz, Ievgeniia Zagoriy, Bernhard Hampoelz, Xiaojie Zhang, Philipp S. Erdmann, Janina Baumbach, Christoph W. Müller, Martin Beck, Jürgen M. Plitzko, Julia Mahamid   +11 more
openaire   +1 more source

Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ

eLife, 2023
Nuclear processes depend on the organization of chromatin, whose basic units are cylinder-shaped complexes called nucleosomes. A subset of mammalian nucleosomes in situ (inside cells) resembles the canonical structure determined in vitro 25 years ago ...
Zhi Yang Tan, Shujun Cai, Alex J Noble, Jon K Chen, Jian Shi, Lu Gan   +5 more
doaj   +1 more source

Advanced cryo-tomography workflow developments - correlative microscopy, milling automation and cryo-lift-out [PDF]

, 2020
Cryo-electron tomography (cryo-ET) is a groundbreaking technology for 3D visualisation and analysis of biomolecules in the context of cellular structures. It allows structural investigations of single proteins as well as their spatial arrangements within
Erdmann, P., Goetze, B., Kuba, J., Rigort, A., Berka, L., Kirmse, R., Mitchels, J., De Bock, J., Hovorka, M., König, J.   +9 more
core   +1 more source

Assembly and Imaging Set up of PIE-Scope

Bio-Protocol, 2020
Cryo-Electron Tomography (cryo-ET) is a method that enables resolving the structure of macromolecular complexes directly in the cellular environment. However, sample preparation for in situ Cryo-ET is labour-intensive and can require both cryo-lamella ...
Sergey Gorelick, David Dierickx, Genevieve Buckley, James Whisstock, Alex Marco   +4 more
doaj   +1 more source

Waffle Method: A general and flexible approach for improving throughput in FIB-milling

Nature Communications, 2022
Here the authors describe the Waffle Method, aimed at increasing the throughput of and solves several challenges present in cryo-FIB/SEM sample preparation for cryo-ET analysis — the highest-resolution method for obtaining 3D views of native biological ...
Kotaro Kelley, Ashleigh M. Raczkowski, Oleg Klykov, Pattana Jaroenlak, Daija Bobe, Mykhailo Kopylov, Edward T. Eng, Gira Bhabha, Clinton S. Potter, Bridget Carragher, Alex J. Noble   +10 more
doaj   +1 more source