Results 31 to 40 of about 177,867 (249)

Integrated Fluorescence Microscopy (iFLM) for Cryo-FIB-milling and In-situ Cryo-ET

bioRxiv, 2023
Correlative cryo-FLM-FIB milling is a powerful sample preparation technique for in situ cryo-ET. However, correlative workflows that incorporate precise targeting remain challenging.
Jae E Yang, Veronika Vrbovská, Tilman Franke, Bryan S. Sibert, Matthew R. Larson, Alexander S Hall, Alex Rigort, John Mitchels, Elizabeth R. Wright   +8 more
semanticscholar   +3 more sources

An improved cryo-FIB method for fabrication of frozen hydrated lamella

Journal of Structural Biology, 2016
Cryo-electron tomography (cryo-ET) provides great insights into the ultrastructure of cells and tissues in their native state and provides a promising way to study the in situ 3D structures of macromolecular complexes. However, this technique has been limited on the very thin specimen, which is not applicable for most cells and tissues.
Fei Sun, Gang Ji, Xiaojun Huang
exaly   +3 more sources

Ultrastructure of human brain tissue vitrified from autopsy revealed by cryo-ET with cryo-plasma FIB milling

Nature Communications
Ultrastructure of human brain tissue has traditionally been examined using electron microscopy (EM) following fixation, staining, and sectioning, which limit resolution and introduce artifacts.
Benjamin C. Creekmore, Kathryn Kixmoeller, Ben E. Black, Edward B. Lee, Yi-Wei Chang   +4 more
doaj   +2 more sources

Labeling and Localization Strategies for In Situ Cryo-Electron Tomography Across the Viral Life Cycle [PDF]

Viruses
Cryo-electron tomography (Cryo-ET) has emerged as a transformative tool for visualizing viral components within their native cellular environment, enabling structural interrogation of viral life cycle events at nanometer resolution without chemical ...
Yoon Ho Park, Rana Kim, Kun-Ho Song, Hyun Suk Jung   +3 more
doaj   +2 more sources

Ice‐Droplet and Damaged Elastomer Interfaces: A Closer Look at Contact and Morphology of Anti‐Icing Coatings Using Cryo‐ FIB ‐ SEM

Journal of Applied Polymer Science, EarlyView.
Silicone elastomers have been identified as promising anti‐icing coatings due to their low ice adhesion strength. However, their softness has led to concerns about the durability of their icephobicity.
Catherine M. Megregian, Fraser H. J. Laidlaw, Vasileios Koutsos, Jane R. Blackford   +3 more
semanticscholar   +2 more sources

Correlative Cryo-FIB and Cryo-ET of Dendritic Spines and Synaptic Connections. [PDF]

Microsc Microanal, 2023
Anderson ED, Cronkite CA, Tolias KF, Ludtke SJ.   +3 more
europepmc   +3 more sources

Cryo-FIB preparation of whole cells and tissue for cryo-TEM: use of high-pressure frozen specimens in tubes and planchets. [PDF]

J Microsc, 2021
The desire to study macromolecular complexes within their cellular context requires the ability to produce thin samples suitable for cryo-TEM (cryo-transmission electron microscope) investigations.
DE Winter DAM, Hsieh C, Marko M, Hayles MF.   +3 more
europepmc   +2 more sources

Accurate structural characterization of nanopores in coal by cryo-FIB-SEM

Advances in Geo-Energy Research
Gang Wang, Xuechang Chen, Houquan Zhang, Jing Wang, Hao Xu   +4 more
semanticscholar   +2 more sources

Focused Ion Beam Milling and Cryo-electron Tomography Methods to Study the Structure of the Primary Cell Wall in Allium cepa

Bio-Protocol, 2022
Cryo-electron tomography (cryo-ET) is a formidable technique to observe the inner workings of vitrified cells at a nanometric resolution in near-native conditions and in three-dimensions. One consequent drawback of this technique is the sample thickness,
William Nicolas, Grant Jensen, Elliot Meyerowitz   +2 more
doaj   +1 more source

A cryogenic, coincident fluorescence, electron, and ion beam microscope

eLife, 2022
Cryogenic electron tomography (cryo-ET) combined with subtomogram averaging, allows in situ visualization and structure determination of macromolecular complexes at subnanometre resolution.
Daan B Boltje, Jacob P Hoogenboom, Arjen J Jakobi, Grant J Jensen, Caspar TH Jonker, Max J Kaag, Abraham J Koster, Mart GF Last, Cecilia de Agrela Pinto, Jürgen M Plitzko, Stefan Raunser, Sebastian Tacke, Zhexin Wang, Ernest B van der Wee, Roger Wepf, Sander den Hoedt   +15 more
doaj   +1 more source