Particle sizing in milk by combined differential dynamic microscopy and cryo-FIB-SEM tomography [PDF]
Soft MatterMilk is a suspension with a multimodal size distribution of fat droplets and protein micelles, which most sizing methods do not distinguish. We demonstrate the use of differential dynamic microscopy (DDM) and cryo-FIB-SEM tomography to size both fat ...
J. J. Bradley +9 more
semanticscholar +4 more sources
Cryo‐FIB Lift‐Out and Electron Tomography Workflow for Bacteria–Nanopillar Interface Imaging Under Native Conditions: Investigating Dragonfly Inspired Bactericidal Titanium Surfaces [PDF]
Small MethodsDragonfly- and cicada-wing-inspired titanium nanostructured surfaces exhibit promising bactericidal properties. However, direct visualisation of the bacteria–material interface under hydrated conditions remains limited, restricting experimental ...
Chaturanga D. Bandara +7 more
semanticscholar +3 more sources
An introduction to cryo-FIB-SEM cross-sectioning of frozen, hydrated Life Science samples [PDF]
Journal of Microscopy, 2021 The introduction of cryo-techniques to the focused ion-beam scanning electron microscope (FIB-SEM) has brought new opportunities to study frozen, hydrated samples from the field of Life Sciences.
Michael Hayles, Matthijs de Winter
exaly +4 more sources
Ice adhesion on hydrophilic and hydrophobic surfaces: Visualization with Cryo-FIB-SEM
Results in EngineeringIce adhesion on solid surfaces poses significant challenges in aviation, infrastructure, and marine applications, particularly in cold climates. Understanding the interfacial bonding mechanisms between ice and different substrate types is crucial for ...
Hassan Abbas Khawaja +3 more
doaj +3 more sources
In situ fiducial markers for 3D correlative cryo-fluorescence and FIB-SEM imaging
iScience, 2021 Summary: Imaging of cells and tissues has improved significantly over the last decade. Dual-beam instruments with a focused ion beam mounted on a scanning electron microscope (FIB-SEM), offering high-resolution 3D imaging of large volumes and fields-of ...
Nadav Scher +3 more
doaj +2 more sources
Examining atherosclerotic lesions in three dimensions at the nanometer scale with cryo-FIB-SEM [PDF]
Proc Natl Acad Sci U S A, 2022 Significance Cholesterol crystallization is crucial in the development of atherosclerosis. Cholesterol crystals contribute to the rupture of the atherosclerotic plaque, which can result in myocardial infarction and stroke.
Jenny Capua-Shenkar +9 more
semanticscholar +2 more sources
Communications Biology, 2023 Cryo-focused ion beam (cryo-FIB) milling technology has been developed for the fabrication of cryo-lamella of frozen native specimens for study by in situ cryo-electron tomography (cryo-ET).
Shuoguo Li +8 more
doaj +2 more sources
PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy [PDF]
eLife, 2019 Cryo-electron tomography (cryo-ET) is emerging as a revolutionary method for resolving the structure of macromolecular complexes in situ. However, sample preparation for in situ Cryo-ET is labour-intensive and can require both cryo-lamella preparation ...
Sergey Gorelick +8 more
doaj +2 more sources
Isolation, cryo-laser scanning confocal microscope imaging and cryo-FIB milling of mouse glutamatergic synaptosomes. [PDF]
PLoS ONE, 2022 Ionotropic glutamate receptors (iGluRs) at postsynaptic terminals mediate the majority of fast excitatory neurotransmission in response to release of glutamate from the presynaptic terminal.
Prerana Gogoi +2 more
doaj +2 more sources
The Potential of Subsampling and Inpainting for Fast Low-Dose Cryo FIB-SEM Imaging. [PDF]
Microscopy and Microanalysis, 2023 Traditional image acquisition for cryo focused ion-beam scanning electron microscopy (FIB-SEM) tomography often sees thousands of images being captured over a period of many hours, with immense data sets being produced.
D. Nicholls +9 more
semanticscholar +2 more sources