Results 21 to 30 of about 177,867 (249)
Novel combination of integrated FLM with cryo-FIB allows targeted cryo-ET sample preparation from challenging samples [PDF]
BIO Web of ConferencesCryo-electron tomography (cryo-ET) has revolutionized structural biology by enabling the observation and characterization of biological samples at a molecular level in their near-native state [1].
Smeets Marit +5 more
doaj +2 more sources
Practical Approaches for Cryo-FIB Milling and Applications for Cellular Cryo-Electron Tomography. [PDF]
Methods Mol Biol, 2021 Cryo-electron tomography (cryo-ET) is a powerful technique to examine cellular structures as they exist in situ. However, direct imaging by TEM for cryo-ET is limited to specimens up to ∼400 nm in thickness, narrowing its applicability to areas such as cellular projections or small bacteria and viruses.
Lam V, Villa E.
europepmc +4 more sources
Precise light and fluorescent microscopy guided sequential cryo FIB lift-out [PDF]
BIO Web of ConferencesCryo-TEM tomography is a well-established imaging technique for revealing intracellular ultrastructure at the level of individual molecules. However, the requirement for electron transparency limited what could be imaged.
Pinkas Dominik +4 more
doaj +2 more sources
Analytical Chemistry, 2022 The development of instruments combining multiple characterization and imaging tools drove huge advances in material science, engineering, biology, and other related fields. Notably, the coupling of SEM with micro-Raman spectrometry (μRaman) provides the
Mouad Essani +9 more
semanticscholar +2 more sources
Cryo-focused Ion Beam Sample Preparation for Imaging Vitreous Cells by Cryo-electron Tomography [PDF]
Bio-Protocol, 2015 Cryo-electron tomography (CET) is a well-established technique for imaging cellular and molecular structures at sub-nanometer resolution. As the method is limited to samples that are thinner than 500 nm, suitable sample preparation is required to attain ...
Miroslava Schaffer +5 more
doaj +2 more sources
In situ protein micro-crystal fabrication by cryo-FIB for electron diffraction. [PDF]
Biophys Rep, 2018 Micro-electron diffraction (MicroED) is an emerging technique to use cryo-electron microscope to study the crystal structures of macromolecule from its micro-/nano-crystals, which are not suitable for conventional X-ray crystallography. However, this technique has been prevented for its wide application by the limited availability of producing good ...
Li X, Zhang S, Zhang J, Sun F.
europepmc +4 more sources
Cryo-FIB-SEM as a promising tool for localizing proteins in 3D
Journal of Structural Biology, 2020 Focused Ion Beam-Scanning Electron Microscopy (FIB-SEM) is an invaluable tool to visualize the 3D architecture of cell constituents and map cell networks. Recently, amorphous ice embedding techniques have been associated with FIB-SEM to ensure that the biological material remains as close as possible to its native state.
Luca Bertinetti +2 more
exaly +5 more sources
Motion-corrected Noise2Noise denoising for structural analysis of cells and tissues with (cryo-)FIB/SEM [PDF]
BioinformaticsMotivation (Cryo-)FIB/SEM enables 3D ultrastructural analysis of cells and tissues at nanoscale resolution. However, its characteristically low signal-to-noise ratio severely hinders interpretation of the volume data.
V. González-Ruiz, J. Fernández
semanticscholar +2 more sources
Cryo‐FIB‐lift‐out: practically impossible to practical reality [PDF]
Journal of Microscopy, 2020 SummaryIn this paper, we explore the development of the Cryo‐Lift‐Out (cryo‐LO) technique as preparation tool for cryogenic transmission electron microscopy (cryo‐TEM). What started in early work defying what was considered ‘practically impossible’ has developed into state‐of‐the‐art practical reality.
CHRISTOPHER DAVID PARMENTER +1 more
openaire +4 more sources
Skeletal muscle triad junction ultrastructure by Focused-Ion-Beam milling of muscle and Cryo-Electron Tomography [PDF]
European Journal of Translational Myology, 2015 Cryo-electron tomography (cryo-ET) has emerged as perhaps the only practical technique for revealing nanometer-level three-dimensional structural details of subcellular macromolecular complexes in their native context, inside the cell.
Terence Wagenknecht +2 more
doaj +2 more sources