Results 51 to 60 of about 177,867 (249)

Preparing Arabidopsis thaliana root protoplasts for cryo electron tomography

Frontiers in Plant Science, 2023
The use of protoplasts in plant biology has become a convenient tool for the application of transient gene expression. This model system has allowed the study of plant responses to biotic and abiotic stresses, protein location and trafficking, cell wall ...
Ingrid Berenice Sanchez Carrillo, Patrick C. Hoffmann, Teura Barff, Martin Beck, Martin Beck, Hugo Germain   +5 more
doaj   +1 more source

Site-Specific Cryo-focused Ion Beam Sample Preparation Guided by 3D Correlative Microscopy. [PDF]

, 2016
The development of cryo-focused ion beam (cryo-FIB) for the thinning of frozen-hydrated biological specimens enabled cryo-electron tomography (cryo-ET) analysis in unperturbed cells and tissues.
Plitzko, J., Mahamid, J., Marco, A., Arnold, J., Lucic, V., Hyman, A., Baumeister, W., Fernandez, J., Laugks, T., Mayer, T.   +9 more
core   +2 more sources

Cryo-FIB workflow for imaging brain tissue via in situ cryo-electron microscopy

bioRxiv, 2023
Cryo-electron microscopy (cryo-EM) enables the study of protein complexes, cytoskeletal elements, and organelles in three dimensions without the use of chemical fixation.
J. Ning, J. Glausier, C. Hsieh, Thomas Schmelzer, Silas A. Buck, J. Franks, Cheri M. Hampton, David A. Lewis, M. Marko, Z. Freyberg   +9 more
semanticscholar   +1 more source

Cryo-FIB Milling protocol for mammalian cells v1

, 2023
Cryo-Electron Tomography (Cryo-ET) and Cryo-Focused Ion Beam (Cryo-FIB) milling provides insight into the structure and architecture of various proteins and organelles in cells. Mammalian cells require to be thinned into sections of ~150-200nm to gain high-resolution information about the contents of the cell.
Josh Hutchings, Tamar Basiashvili, Elizabeth Villa   +2 more
openaire   +1 more source

Cryo-plasma FIB/SEM volume imaging of biological specimens

eLife, 2023
Serial focussed ion beam scanning electron microscopy (FIB/SEM) enables imaging and assessment of subcellular structures on the mesoscale (10 nm to 10 µm). When applied to vitrified samples, serial FIB/SEM is also a means to target specific structures in
Maud Dumoux, Thomas Glen, Jake LR Smith, Elaine ML Ho, Luis MA Perdigão, Avery Pennington, Sven Klumpe, Neville BY Yee, David Andrew Farmer, Pui YA Lai, William Bowles, Ron Kelley, Jürgen M Plitzko, Liang Wu, Mark Basham, Daniel K Clare, C Alistair Siebert, Michele C Darrow, James H Naismith, Michael Grange   +19 more
doaj   +1 more source

Mind the corner: Fillets in cryo-FIB lamella preparation to minimise sample loss caused by stress concentration and lamella breakage

bioRxiv
Cryo-FIB milling of biological specimens is a critical and limiting step in the cryo-electron tomography workflow. Preparing electron-transparent cryo-lamellae is a serial, low-throughput process.
Sergey Gorelick, Sailakshmi Velamoor, Patrick Cleeve, S. Trépout, Le Ying, V. Naranbhai, G. Ramm   +6 more
semanticscholar   +1 more source

Bringing Structure to Cell Biology with Cryo-Electron Tomography [PDF]

, 2023
Recent advances in cryo-electron microscopy have marked only the beginning of the potential of this technique. To bring structure into cell biology, the modality of cryo-electron tomography has fast developed into a bona fide in situ structural biology ...
Young, Lindsey, Elizabeth Villa, Lindsey N. Young, Villa, Elizabeth   +3 more
core   +1 more source

A streamlined workflow for automated cryo focused ion beam milling

, 2021
Cryo-electron tomography (cryo-ET) is an emerging technique to study the cellular architecture and the structure of proteins at high resolution in situ.
Plitzko, J., Erdmann, P., Grange, M., Tacke, S., Raunser, S., Klumpe, S., Wang, Z., Stefan Raunser, Zhexin Wang, Michael Grange, Sebastian Tacke, Sven Klumpe, Philipp Erdmann, Jürgen Plitzko   +13 more
core   +1 more source

Application of cryo-FIB-SEM for investigating ultrastructure in guard cells of higher plants.

Plant physiology and biochemistry : PPB
Stomata are vital for CO2 and water vapor exchange, with guard cells' aperture and ultrastructure highly responsive to environmental cues. However, traditional methods for studying guard cell ultrastructure, which rely on chemical fixation and embedding,
B. L. Franzisky, Xudong Zhang, Claus Jakob Burkhardt, Endre Majorovits, Eric Hummel, Andreas Schertel, C. Geilfus, C. Zörb   +7 more
semanticscholar   +1 more source

Direct Visualization of Cellular Protein Complexes in situ by Fluorescence-Guided Cryo-FIB-SEM and Cryo-ET [PDF]

Cryogenic electron tomography (cryo-ET) is a technique that can reconstruct three-dimensional volumes of large protein complexes in situ at sub-nanometer resolution.
Wang, Jue (Phyllis)
core   +1 more source