Results 61 to 70 of about 177,867 (249)

Advancing Cryo-ET Sample Preparation: Integrated FLM for Cryo-FIB for targeted milling of challenging samples.

Structural Dynamics
Cryo-electron tomography (cryo-ET) has transformed structural biology, offering unparalleled insights into biological samples at near-native state [1]. However, its efficacy relies on thin specimens, typically below 200 nm, achievable through Cryogenic ...
Samuel Záchej, Wessel Teunisse, Marit Smeets, Jakub Javůrek, Dominik Pinkas, Vlada Filimonenko   +5 more
semanticscholar   +1 more source

3D Cryo-FIB SEM - Mallomonas Dataset

, 2019
Dataset of raw and image processed 3D Cryo-FIB SEM data recorded from Mallomonas ...
Weiss, Ingrid M., Schertel, Andreas, Lemloh, Marie-Louise, Hörning, Marcel, Schneider, Ralf, Schweikert, Michael R.   +5 more
core   +1 more source

Correlative Light and Electron Cryo-Microscopy Workflow Combining Micropatterning, Ice Shield, and an In-Chamber Fluorescence Light Microscope

Bio-Protocol, 2023
In situ cryo-electron tomography (cryo-ET) is the most current, state-of-the-art technique to study cell machinery in its hydrated near-native state. The method provides ultrastructural details at sub-nanometer resolution for many components within the ...
Sabrina Berkamp, Deniz Daviran, Marit Smeets, Alexane Caignard, Riddhi Jani, Pia Sundermeyer, Caspar Jonker, Sven Gerlach, Bernd Hoffmann, Katherine Lau, Carsten Sachse   +10 more
doaj   +1 more source

Sample Preparation by 3D-Correlative Focused Ion Beam Milling for High-Resolution Cryo-Electron Tomography

, 2021
Cryo-electron tomography (cryo-ET) has become the method of choice for investigating cellular ultrastructure and molecular complexes in their native, frozen-hydrated state.
Wilfling, F., Plitzko, J., Erdmann, P., Wilfling, F. ; https://orcid.org/, Capitanio, C., Bieber, A., Cristina Capitanio, Florian Wilfling, Anna Bieber, Philipp S. Erdmann, Jürgen Plitzko   +10 more
core   +1 more source

Endocytosed nanogold fiducials for improved in-situ cryo-electron tomography tilt-series alignment [PDF]

, 2021
Cryo-electron tomography (CET) on cryo-focused ion beam (FIB)-milled lamellae is becoming a powerful technique for determining the structure of macromolecular complexes in their native cellular environment.
Ravelli, Raimond B. G., Peters, Peter J.; id_orcid, Lopez-Iglesias, Carmen, Berger, Casper, Carmen López-Iglesias, Casper Berger, Peter J. Peters, Raimond B.G. Ravelli   +7 more
core   +1 more source

Architecting Phase‐Selective Interlayers for Stable SiOx Lithium Storage via Dual‐Function Design

Advanced Science, EarlyView.
Silicon anodes offer high capacity but suffer severe volume expansion. Silicon monoxide (SiO) buffers expansion, yet uncontrolled lithiation irreversibly consumes lithium as Li2O and oxygen‐rich silicates. Here, we design a layered SiOx featuring a phase‐selective conductive interfacial interlayer (PSCII), i.e., a Li4SiO4‐rich, ion‐accessible ...
Zhenfei Chang, Wei Dong, Yingguang Zhang, Hangchen Qu, Yifei Wang, Xiaolong Zhao, Chu Wang, Ming Dong, Huizhi Wang, Jin Xuan, Yug Joshi, Dennis Y.C. Leung, Wending Pan   +12 more
wiley   +1 more source

A method for restoring signals and revealing individual macromolecule states in cryo-ET, REST

Nature Communications, 2023
Cryo-electron tomography (cryo-ET) is widely used to explore the 3D density of biomacromolecules. However, the heavy noise and missing wedge effect prevent directly visualizing and analyzing the 3D reconstructions.
Haonan Zhang, Yan Li, Yanan Liu, Dongyu Li, Lin Wang, Kai Song, Keyan Bao, Ping Zhu   +7 more
doaj   +1 more source

A Multimodal Piezoelectric Vertical Sidewall Architecture for Scalable In‐Plane NEMS Resonators

Advanced Electronic Materials, EarlyView.
A vertical in‐plane piezoelectric resonator design shifts frequency tuning from film thickness to lithographic lateral definition, facilitating monolithic multiband integration. Initially validated via microscale length‐extensional resonators, this cavities‐first fabrication approach scales to the nanoscale for high‐frequency operation.
Artem Gabrelian, Kristina Bespalova, Glenn Ross, Mervi Paulasto‐Kröckel   +3 more
wiley   +1 more source

Extracellular filaments revealed by affinity capture cryogenic-electron tomography

Nature Communications
Cryogenic-electron tomography (cryo-ET) has provided an unprecedented glimpse into the nanoscale architecture of cells by combining cryogenic preservation of biological structures with electron tomography. Micropatterning of extracellular matrix proteins
Leeya Engel, Magda Zaoralová, Momei Zhou, Alexander R. Dunn, Stefan L. Oliver   +4 more
doaj   +1 more source

Cryo-FIB-preparation and cryo-SEM investigation of gold nanolayers used as absorber for laser welding of polymer foils

, 2022
S.11-18The morphology of welded polymer foils was investigated by Scanning Electron Microscopy (SEM) using Focused Ion Beam (FIB) technology for cross-sectional preparation.
Neuber, M., Heilmann, A., Cismak, A.
core   +1 more source