Results 21 to 30 of about 56,400 (87)

Correlative Light and Electron Microscopy in Cell Biology: Accessible Pre-Embedding and Post-Embedding Strategies

Image Analysis and Stereology
Correlative light and electron microscopy (CLEM) combines the molecular specificity of fluorescence light microscopy with the ultrastructural resolution of electron microscopy (EM), enabling molecularly defined cellular structures to be localised and ...
Aleksandar Janev, Urška Dragin Jerman, Mateja Erdani Kreft   +2 more
doaj   +1 more source

Multi‐Scale Investigation of Supramolecular Organogels Assembled From Pt(II)‐Salphen Complexes

Chemistry – A European Journal, EarlyView.
A family of Pt(II)‐salphen complexes with long alkyl chains is investigated as low‐molecular‐weight organogelators. Multi‐scale characterization of their supramolecular gels reveals how the chain length, Pt‐Pt interactions, and π–π stacking direct self‐assembly and tune gel mechanics.
Pablo Msellem, Davide Condotta, Lise‐Marie Chamoreau, Mickaël Pomes‐Hadda, Nathan J. Van Zee, Cédric Lorthioir, Clément Guibert, Guillaume Vives   +7 more
wiley   +1 more source

HPM live μ for a full CLEM workflow

, 2021
With the development of advanced imaging methods that took place in the last decade, the spatial correlation of microscopic and spectroscopic information-known as multimodal imaging or correlative microscopy (CM)-has become a broadly applied technique to
Heiligenstein, X., Schmitt, F., Salamero, J., Lamers, E., Lindenau, J., Eyraud, F., Sommerdijk, N., Beer, M.A. de, Kea-te Lindert, M.M., Heiligenstein, J., Akiva, A., Manet, L., Raposo, G., Belle, M., Nico Sommerdijk, Laurent Manet, Fabrice Schmitt, Marit de Beer, Jean Salamero, Frédérique Eyraud, Edwin Lamers, Martin Belle, Xavier Heiligenstein, Jérôme Heiligenstein, Mariska Kea-te Lindert, Graça Raposo, Anat Akiva, Joerg Lindenau   +27 more
core   +1 more source

Investigating the Cytoskeleton of DRGs Using Cryo‐Electron Microscopy and Deep Learning

Cytoskeleton, EarlyView.
ABSTRACT We describe a method for determining the ultrastructural organization of axons and varicosities of cultured dorsal root ganglion (DRG) neurons using cryogenic electron microscopy (cryo‐EM). Cryo‐EM reveals the dimensions, proximity, and overall organization of biological specimens in a near‐native state, avoiding artifacts of fixation and ...
M. Neal Waxham, Mihir Relan, Matthew T. Swulius, Andrea K. H. Stavoe   +3 more
wiley   +1 more source

Glucose starvation triggers filamentous septin assemblies in an S. pombe septin-2 deletion mutant

Biology Open, 2019
Using correlative light and electron microscopy (CLEM), we studied the intracellular organization by of glucose-starved fission yeast cells (Schizosaccharomyces pombe) with regards to the localization of septin proteins throughout the cytoplasm. Thereby,
Minghua Liu, Maria B. Heimlicher, Mirjam Bächler, Chieze C. Ibeneche-Nnewihe, Ernst-Ludwig Florin, Damian Brunner, Andreas Hoenger   +6 more
doaj   +1 more source

Rapid Fabrication of Sub‐100 µm Microstructures In Situ via Femtosecond Laser for Correlative Imaging of Rare Cells

Small, EarlyView.
In this work, a femtosecond (fs) laser processing strategy is demonstrated for rapid and targeted fabrication of 3D sub‐100‐µm microstructures on resin‐embedded cells. The fabricated array of sub‐100‐µm pillars (each loaded with flow‐sorted T‐cell subsets) is arranged in a half‐grid geometry to enable efficient use of the scarce biological sample and ...
Aristide Djoulde, Yaping Chen, Vahid R. Adineh, Yang Liu, Boyin Liu, Di Yu, Jing Fu   +6 more
wiley   +1 more source

The autophagy research in electron microscopy

Applied Microscopy, 2019
Autophagy, a highly conserved process of eukaryotic cellular recycling, plays an important role in cell survival and maintenance. Dysfunctional autophagy contributes to the pathologies of many human diseases.
Minkyo Jung, Hyosun Choi, Ji Young Mun
doaj   +1 more source

Advances in high‐resolution cryo‐volume electron microscopy (cvEM) imaging for unicellular and multicellular organisms

Journal of Microscopy, EarlyView.
Abstract Cryo‐Focused Ion Beam Scanning Electron Microscopy (cryoFIB‐SEM) using samples fixed by high‐pressure freezing uniquely enables high‐resolution cryo‐volume Electron Microscope (cvEM) images of cell ultrastructure to be obtained from whole cells and complex tissues in their near native state. As the freezing process also preserves fluorescence,
Maryna Kobylynska, Daniel Nicholls, Zoe Broad, Jack Wells, Alex Robinson, Stefania Marcotti, Damien McGrouther, Ch'ng Q‐L, Genoveva Esteban, Nigel Browning, Roland Fleck   +10 more
wiley   +1 more source

Fluorescence-Based Detection of Membrane Fusion State on a Cryo-EM Grid using Correlated Cryo-Fluorescence and Cryo-Electron Microscopy

, 2019
Correlated light and electron microscopy (CLEM) has become a popular technique for combining the protein-specific labeling of fluorescence with electron microscopy, both at room and cryogenic temperatures.
Briggs, J., Metskas, L.
core   +1 more source

Labeling and Localization Strategies for In Situ Cryo-Electron Tomography Across the Viral Life Cycle

Viruses
Cryo-electron tomography (Cryo-ET) has emerged as a transformative tool for visualizing viral components within their native cellular environment, enabling structural interrogation of viral life cycle events at nanometer resolution without chemical ...
Yoon Ho Park, Rana Kim, Kun-Ho Song, Hyun Suk Jung   +3 more
doaj   +1 more source