Results 21 to 30 of about 2,441,948 (283)
The promise and the challenges of cryo‐electron tomography
FEBS Letters, 2020 Structural biologists have traditionally approached cellular complexity in a reductionist manner in which the cellular molecular components are fractionated and purified before being studied individually. This ‘divide and conquer’ approach has been highly successful.
Martin Turk, Wolfgang Baumeister
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Characterization of insulin crystalline form in isolated β-cell secretory granules
Open Biology, 2022 Insulin is stored in vivo inside the pancreatic β-cell insulin secretory granules. In vitro studies have led to an assumption that high insulin and Zn2+ concentrations inside the pancreatic β-cell insulin secretory granules should promote insulin ...
Seiya Asai +7 more
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Pathogen-host reorganization during Chlamydia invasion revealed by cryo-electron tomography [PDF]
, 2014 Invasion of host cells is a key early event during bacterial infection, but the underlying pathogen-host interactions are yet to be fully visualised in three-dimensional detail.
Hayward, Richard +5 more
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In situ structure and dynamics of an alphacoronavirus spike protein by cryo-ET and cryo-EM
Nature Communications, 2022 Hsu and co-workers integrate cryo-electron tomography, cryo-electron microscopy and mass spectrometry to reveal the structural polymorphism of a pig coronavirus spike protein within intact viral particles, and how glycosylation modulates the ...
Cheng-Yu Huang +14 more
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Cryo-electron tomography of vaccinia virus [PDF]
Proceedings of the National Academy of Sciences, 2005 The combination of cryo-microscopy and electron tomographic reconstruction has allowed us to determine the structure of one of the more complex viruses, intracellular mature vaccinia virus, at a resolution of 4–6 nm. The tomographic reconstruction allows us to dissect the different structural components of the viral particle, avoiding projection ...
Cyrklaff, M. +6 more
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How to build a ribosome from RNA fragments in Chlamydomonas mitochondria
Nature Communications, 2021 Mitoribosomes are remarkably diverse in their structures and compositions. Here the authors combine biochemistry, genetics, single particle cryo-electron microscopy and in situ cryo-electron tomography to reveal the mitochondrial ribosome of ...
Florent Waltz +11 more
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High-throughput high-resolution cryo-electron crystallography [PDF]
, 2015 High-resolution structures of membrane and soluble proteins can be obtained by cryo-electron crystallography, given highly-ordered cryo-preparations of perfectly flat 2D crystals are available.
Scherer, Sebastian
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Cryo-Electron Tomography of Rubella Virus [PDF]
Journal of Virology, 2012 ABSTRACT Rubella virus is the only member of the Rubivirus genus within the Togaviridae family and is the causative agent of the childhood disease known as rubella or German measles.
Anthony J, Battisti +8 more
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Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress
Nature Communications, 2021 In this study, Peijun Zhang and colleagues use cryoFIB/SEM volume imaging and soft x-ray cryo-tomography with cryo-electron tomography (cryoET) of cellular periphery, lamellae, and subtomogram averaging to place critical structural events in the SARS-CoV-
Luiza Mendonça +19 more
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3D ultrastructural organization of whole Chlamydomonas reinhardtii cells studied by nanoscale soft x-ray tomography [PDF]
, 2012 The complex architecture of their structural elements and compartments is a hallmark of eukaryotic cells. The creation of high resolution models of whole cells has been limited by the relatively low resolution of conventional light microscopes and the ...
Achilleas S. Frangakis +40 more
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