Results 1 to 10 of about 12,872 (110)
Quantitative Cryo-Electron Tomography
Frontiers in Molecular Biosciences, 2022 The three-dimensional organization of biomolecules important for the functioning of all living systems can be determined by cryo-electron tomography imaging under native biological contexts. Cryo-electron tomography is continually expanding and evolving,
Paula P. Navarro, Paula P. Navarro
doaj +3 more sources
An image processing pipeline for electron cryo‐tomography in RELION‐5 [PDF]
FEBS Open BioElectron tomography of frozen, hydrated samples allows structure determination of macromolecular complexes that are embedded in complex environments.
Alister Burt +8 more
doaj +3 more sources
The resolution revolution revisited [PDF]
IUCrJIn this issue of IUCrJ, Subramaniam, Kühlbrandt & Henderson present an overview of the remarkable progress that has been made in electron cryo-microscopy and electron cryo-tomography.
Edward H. Egelman
doaj +2 more sources
Labeling and Localization Strategies for In Situ Cryo-Electron Tomography Across the Viral Life Cycle [PDF]
VirusesCryo-electron tomography (Cryo-ET) has emerged as a transformative tool for visualizing viral components within their native cellular environment, enabling structural interrogation of viral life cycle events at nanometer resolution without chemical ...
Yoon Ho Park +3 more
doaj +2 more sources
Ultra-rapid cryo-EM data acquisition method enabled by continuous recording based beam image shift [PDF]
Nature CommunicationsCryo-electron microscopy (cryo-EM) data acquisition is time-intensive given that a large amount of data is required to obtain a high-resolution reconstruction. Here, we eliminate camera-induced delay time by continuously recording during beam-image shift
Qi Yang +4 more
doaj +2 more sources
Cryo electron tomography of native HIV-1 budding sites. [PDF]
PLoS Pathogens, 2010 The structure of immature and mature HIV-1 particles has been analyzed in detail by cryo electron microscopy, while no such studies have been reported for cellular HIV-1 budding sites.
Lars-Anders Carlson +6 more
doaj +1 more source
Journal of Structural Biology: X, 2022 Advancements in the field of cryo-electron tomography have greatly contributed to our current understanding of prokaryotic cell organization and revealed intracellular structures with remarkable architecture.
Janine Liedtke +2 more
doaj +1 more source
Preparing Arabidopsis thaliana root protoplasts for cryo electron tomography
Frontiers in Plant Science, 2023 The use of protoplasts in plant biology has become a convenient tool for the application of transient gene expression. This model system has allowed the study of plant responses to biotic and abiotic stresses, protein location and trafficking, cell wall ...
Ingrid Berenice Sanchez Carrillo +5 more
doaj +1 more source
Recent Advances in Cryo Electron Microscopy and Tomography [PDF]
Structural DynamicsIn the recent years, cryo-electron microscopy (cryo-EM) single-particle analysis (SPA) has greatly benefited from a multitude of technological advances cumulating in breaking the atomic-resolution barrier for biological macromolecules.
Eliza Nieweglowska +2 more
doaj +3 more sources
LIMITING FACTORS IN SINGLE PARTICLE CRYO ELECTRON TOMOGRAPHY
Computational and Structural Biotechnology Journal, 2012 Modern methods of cryo electron microscopy and tomography allow visualization of protein nanomachines in their native state at the nanometer scale. Image processing methods including sub-volume averaging applied to repeating macromolecular elements ...
Mikhail Kudryashev +2 more
doaj +3 more sources