Results 41 to 50 of about 2,460,746 (279)

Direct visualization of vaults within intact cells by electron cryo-tomography [PDF]

Cellular and Molecular Life Sciences, 2015
The vault complex is the largest cellular ribonucleoprotein complex ever characterized and is present across diverse Eukarya. Despite significant information regarding the structure, composition and evolutionary conservation of the vault, little is know about the complex's actual biological function.
Woodward, Cora L., Mendonça, Luiza M., Jensen, Grant J.   +2 more
openaire   +3 more sources

Cryo-Electron Tomography of the Mammalian Synapse [PDF]

, 2018
Characterizing the detailed structure of the mammalian synapse is of crucial importance to understand its mechanisms of function. Here I describe a protocol to study synaptic architecture by cryo-electron tomography (cryo-ET), a powerful electron ...
Rubén Fernández-Busnadiego, Fernandez-Busnadiego, R.   +1 more
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Orthobunyavirus ultrastructure and the curious tripodal glycoprotein spike [PDF]

, 2013
The genus <i>Orthobunyavirus</i> within the family <i>Bunyaviridae</i> constitutes an expanding group of emerging viruses, which threaten human and animal health.
McLees, A., Christelle Yeromonahos (413081), Juha T. Huiskonen (191734), David Bitto, David Bitto (413079), Bowden, Thomas A, Elliott, Richard M., Richard M. Elliott (252934), Elliott, Richard, Bowden Thomas A., Yeromonahos, Christelle, Huiskonen, Juha T., Angela McLees (413080), Elliott, Richard M, Christelle Yeromonahos, Pierson, Ted, Huiskonen, J.T., Elliott, R.M., Huiskonen, Juha, Richard M Elliott, McLees, Angela, Bowden, T.A., Bitto, David, Bitto David, Huiskonen, Juha T, Elliott Richard M., Bowden, Thomas A., Thomas A. Bowden (413078), McLees Angela, Bitto, D., Bowden, Thomas, Huiskonen Juha T., Angela McLees, Yeromonahos, C., Thomas A Bowden, Yeromonahos Christelle, Juha T Huiskonen   +36 more
core   +1 more source

Visualizing membrane trafficking through the electron microscope: cryo-tomography of coat complexes [PDF]

Acta Crystallographica Section D Structural Biology, 2019
Coat proteins mediate vesicular transport between intracellular compartments, which is essential for the distribution of molecules within the eukaryotic cell. The global arrangement of coat proteins on the membrane is key to their function, and cryo-electron tomography and subtomogram averaging have been used to study membrane-bound coat proteins ...
Markova, EA, Zanetti, G
openaire   +4 more sources

Three‐dimensional structure of basal body triplet revealed by electron cryo‐tomography [PDF]

The EMBO Journal, 2011
Basal bodies and centrioles play central roles in microtubule (MT)-organizing centres within many eukaryotes. They share a barrel-shaped cylindrical structure composed of nine MT triplet blades. Here, we report the structure of the basal body triplet at 33 Å resolution obtained by electron cryo-tomography and 3D subtomogram averaging.
Li, Sam, Fernandez, Jose-Jesus, Marshall, Wallace F, Agard, David A   +3 more
openaire   +4 more sources

Cryotomography of budding influenza a virus reveals filaments with diverse morphologies that mostly do not bear a genome at their distal end [PDF]

, 2013
Influenza viruses exhibit striking variations in particle morphology between strains. Clinical isolates of influenza A virus have been shown to produce long filamentous particles while laboratory-adapted strains are predominantly spherical.
Swetha Vijayakrishnan, Vijayakrishnan Swetha, Jackson David, Worawit Suphamungmee, David Bhella, Loney Colin, Bhella, D., Swetha Vijayakrishnan (418947), Colin Loney (418948), David Jackson (84936), Frazer J. Rixon (129686), Colin Loney, Suphamungmee Worawit, Rixon, F.J., Jackson, David, Worawit Suphamungmee (418949), Rixon Frazer J., Vijayakrishnan, Swetha, Rixon, Frazer J., Loney, Colin, Bhella David, David Jackson, Loney, C., Suphamungmee, W., Jackson, D., Bhella, David, Vijayakrishnan, S., David Bhella (129684), Suphamungmee, Worawit, Frazer J Rixon   +29 more
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Cryo-Electron Tomography of Reconstituted Biomolecular Condensates

, 2023
The assembly of membraneless compartments by phase separation has recently been recognized as a mechanism for spatial and temporal organization of biomolecules within the cell.
Woodruff, J., Franzmann, T., Mahamid, J., Tollervey, F., Bose, M., Sachweh, J. ; https://orcid.org/, Zhang, X.   +6 more
core   +1 more source

Electron Cryo-Tomography of Cilia-Associated Structures of Rod Photoreceptors [PDF]

Biophysical Journal, 2011
Vertebrate rod photoreceptors sense light with modified primary cilia known as rod outer segments. The regions of the cell surrounding the junction between the inner and outer segments, known as the connecting cilium or transition zone, are the sites of highly active biosynthetic activity, directional trafficking, and sorting of protein and membranes ...
Gilliam, Jared C., Chang, Juan T., Chiu, Wah, Wensel, Theodore G.   +3 more
openaire   +1 more source

Nanoscale characterization of the biomolecular corona by cryo-electron microscopy, cryo-electron tomography, and image simulation

Nature Communications, 2021
Understanding the biomolecular corona is of key importance to nanomedicine. Here, the authors report on cryo-electron and tomographic imaging of the corona formed on model nanoparticles and the 3D reconstruction of the corona to study the distribution ...
Sara Sheibani, Kaustuv Basu, Ali Farnudi, Aliakbar Ashkarran, Muneyoshi Ichikawa, John F. Presley, Khanh Huy Bui, Mohammad Reza Ejtehadi, Hojatollah Vali, Morteza Mahmoudi   +9 more
doaj   +1 more source

New hardware and workflows for semi-automated correlative cryo-fluorescence and cryo-electron microscopy/tomography [PDF]

, 2017
Correlative light and electron microscopy allows features of interest defined by fluorescence signals to be located in an electron micrograph of the same sample.
Gaechter, Leander, Sieckmann, F., Sonnen, A., Briggs, J., Bebeacua, C., Clarke, M., Clarke, Mairi, Bebeacua, Cecilia, Schorb, Martin, Schorb, M., Bykov, Y., Bykov, Yury S., Sonnen, Andreas F.-P., Lihl, Reinhard, Avinoam, Ori, Lihl, R., Avinoam, O., Gaechter, L., Sieckmann, Frank, Briggs, John A.G.   +19 more
core   +1 more source