Results 31 to 40 of about 2,460,746 (279)
Cryo-electron tomography of biological specimens [PDF]
, 2006 Cryo-electron tomography (CET) is an imaging technique capable of visualizing the three-dimensional (3-D) structure of complex viruses, cells, and tissues in hydrated state.
Leis, A. +13 more
core +1 more source
, 2023 Electron tomography in biology is a technique to reconstruct the three-dimensional structure of macro-molecular, sub-cellular or cellular objects by transmission electron microscopy (TEM).
Ishikawa, Takashi (author)
core +1 more source
In situ structure and dynamics of an alphacoronavirus spike protein by cryo-ET and cryo-EM
Nature Communications, 2022 Hsu and co-workers integrate cryo-electron tomography, cryo-electron microscopy and mass spectrometry to reveal the structural polymorphism of a pig coronavirus spike protein within intact viral particles, and how glycosylation modulates the ...
Cheng-Yu Huang +14 more
doaj +1 more source
A cylindrical specimen holder for electron cryo-tomography
Ultramicroscopy, 2014 The use of slab-like flat specimens for electron cryo-tomography restricts the range of viewing angles that can be used. This leads to the "missing wedge" problem, which causes artefacts and anisotropic resolution in reconstructed tomograms. Cylindrical specimens provide a way to eliminate the problem, since they allow imaging from a full range of ...
Palmer, Colin M., Löwe, Jan
openaire +2 more sources
High-throughput high-resolution cryo-electron crystallography [PDF]
, 2015 High-resolution structures of membrane and soluble proteins can be obtained by cryo-electron crystallography, given highly-ordered cryo-preparations of perfectly flat 2D crystals are available.
Scherer, Sebastian
core +1 more source
Automatic localization and identification of mitochondria in cellular electron cryo-tomography using faster-RCNN. [PDF]
BMC Bioinformatics, 2019 Cryo-electron tomography (cryo-ET) enables the 3D visualization of cellular organization in near-native state which plays important roles in the field of structural cell biology. However, due to the low signal-to-noise ratio (SNR), large volume and high content complexity within cells, it remains difficult and time-consuming to localize and identify ...
Li R +10 more
europepmc +6 more sources
How to build a ribosome from RNA fragments in Chlamydomonas mitochondria
Nature Communications, 2021 Mitoribosomes are remarkably diverse in their structures and compositions. Here the authors combine biochemistry, genetics, single particle cryo-electron microscopy and in situ cryo-electron tomography to reveal the mitochondrial ribosome of ...
Florent Waltz +11 more
doaj +1 more source
Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress
Nature Communications, 2021 In this study, Peijun Zhang and colleagues use cryoFIB/SEM volume imaging and soft x-ray cryo-tomography with cryo-electron tomography (cryoET) of cellular periphery, lamellae, and subtomogram averaging to place critical structural events in the SARS-CoV-
Luiza Mendonça +19 more
doaj +1 more source
3D ultrastructural organization of whole Chlamydomonas reinhardtii cells studied by nanoscale soft x-ray tomography [PDF]
, 2012 The complex architecture of their structural elements and compartments is a hallmark of eukaryotic cells. The creation of high resolution models of whole cells has been limited by the relatively low resolution of conventional light microscopes and the ...
Achilleas S. Frangakis +40 more
core +2 more sources
A tail-like assembly at the portal vertex in intact herpes simplex type-1 virions [PDF]
, 2012 Herpes viruses are prevalent and well characterized human pathogens. Despite extensive study, much remains to be learned about the structure of the genome packaging and release machinery in the capsids of these large and complex double-stranded DNA ...
Rixon Frazer J. +23 more
core +1 more source