Results 21 to 30 of about 2,460,746 (279)
Tracing low-level structures in cryo-electron tomography.
PLoS ONECryo-electron tomography is an imaging technique that provides 3D images (tomograms) in situ of cells with sub-nanometer resolution. Typically, the first step in the analysis is to classify the tomogram voxels into different structures, named semantic ...
Pelayo Alvarez Brecht +5 more
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Studying membrane modulation mechanisms by electron cryo-tomography
Current Opinion in Structural Biology, 2022 Membrane modulation is a key part of cellular life. Critical to processes like energy production, cell division, trafficking, migration and even pathogen entry, defects in membrane modulation are often associated with diseases. Studying the molecular mechanisms of membrane modulation is challenging due to the highly dynamic nature of the oligomeric ...
Davide Zabeo, Karen M. Davies
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Visualization of ATP synthase dimers in mitochondria by electron cryo-tomography. [PDF]
J Vis Exp, 2014 Electron cryo-tomography is a powerful tool in structural biology, capable of visualizing the three-dimensional structure of biological samples, such as cells, organelles, membrane vesicles, or viruses at molecular detail. To achieve this, the aqueous sample is rapidly vitrified in liquid ethane, which preserves it in a close-to-native, frozen-hydrated
Davies KM +7 more
europepmc +6 more sources
Perspectives on electron cryo-tomography of vitreous cryo-sections [PDF]
Microscopy, 2011 A major objective of modern structural biology is to appreciate the cellular organization by elucidating the spatial arrangement of macromolecular complexes within a cell. Cryogenic sample preparation, combined with cryo-ultramicrotomy, enables large cells and pieces of biological tissues to be thinned for electron cryo-tomography, which provides a ...
Jason, Pierson +3 more
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Cellular Electron Cryo-Tomography to Study Virus-Host Interactions [PDF]
Annual Review of Virology, 2020 Viruses are obligatory intracellular parasites that reprogram host cells upon infection to produce viral progeny. Here, we review recent structural insights into virus-host interactions in bacteria, archaea, and eukaryotes unveiled by cellular electron cryo-tomography (cryoET).
Quemin, Emmanuelle R. J. +9 more
openaire +3 more sources
Neurons as a model system for cryo-electron tomography. [PDF]
, 2022 Cryo-electron tomography (Cryo-ET) provides unique opportunities to image cellular components at high resolution in their native state and environment.
Lučić, Vladan +5 more
core +2 more sources
Challenges and triumphs in cryo-electron tomography
iScience, 2021 Summary: Cryo-electron tomography has stepped fully into the spotlight. Enthusiasm is high. Fortunately for us, this is an exciting time to be a cryotomographer, but there is still a way to go before declaring victory.
Ryan K. Hylton, Matthew T. Swulius
doaj +1 more source
Model Compression for Faster Structural Separation of Macromolecules Captured by Cellular Electron Cryo-Tomography. [PDF]
Image Anal Recognit, 2018 8 ...
Guo J, Zhou B, Zeng X, Freyberg Z, Xu M.
europepmc +7 more sources
Characterization of insulin crystalline form in isolated β-cell secretory granules
Open Biology, 2022 Insulin is stored in vivo inside the pancreatic β-cell insulin secretory granules. In vitro studies have led to an assumption that high insulin and Zn2+ concentrations inside the pancreatic β-cell insulin secretory granules should promote insulin ...
Seiya Asai +7 more
doaj +1 more source
Pathogen-host reorganization during Chlamydia invasion revealed by cryo-electron tomography [PDF]
, 2014 Invasion of host cells is a key early event during bacterial infection, but the underlying pathogen-host interactions are yet to be fully visualised in three-dimensional detail.
Hayward, Richard +5 more
core +1 more source