Results 11 to 20 of about 2,460,746 (279)
Identifying the assembly pathway of cyanophage inside the marine bacterium using electron cryo-tomography [PDF]
Microbial Cell, 2014 Advances in electron cryo-tomography open up a new avenue to visualize the 3-D internal structure of a single bacterium before and after its infection by bacteriophages in its native environment, without using chemical fixatives, fluorescent dyes or ...
Wei Dai +3 more
doaj +4 more sources
Communications Biology, 2023 Cryo-focused ion beam (cryo-FIB) milling technology has been developed for the fabrication of cryo-lamella of frozen native specimens for study by in situ cryo-electron tomography (cryo-ET).
Shuoguo Li +8 more
doaj +2 more sources
A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0. [PDF]
Elife, 2022 We present a new approach for macromolecular structure determination from multiple particles in electron cryo-tomography (cryo-ET) data sets. Whereas existing subtomogram averaging approaches are based on 3D data models, we propose to optimise a regularised likelihood target that approximates a function of the 2D experimental images.
Zivanov J +11 more
europepmc +8 more sources
Molecular tags for electron cryo-tomography. [PDF]
Emerg Top Life SciElectron cryotomography enables the direct visualisation of biological specimens without stains or fixation, revealing complex molecular landscapes at high resolution. However, identifying specific proteins within these crowded environments is challenging.
Silvester E, Baker LA.
europepmc +4 more sources
Electron cryo-tomography of vestibular hair-cell stereocilia. [PDF]
J Struct Biol, 2019 High-resolution imaging of hair-cell stereocilia of the inner ear has contributed substantially to our understanding of auditory and vestibular function. To provide three-dimensional views of the structure of stereocilia cytoskeleton and membranes, we developed a method for rapidly freezing unfixed stereocilia on electron microscopy grids, which ...
Metlagel Z +12 more
europepmc +6 more sources
Visualization of cytosolic ribosomes on the surface of mitochondria by electron cryo-tomography. [PDF]
EMBO Rep, 2017 We employed electron cryo-tomography to visualize cytosolic ribosomes on the surface of mitochondria. Translation-arrested ribosomes reveal the clustered organization of the TOM complex, corroborating earlier reports of localized translation. Ribosomes are shown to interact specifically with the TOM complex, and nascent chain binding is crucial for ...
Gold VA +3 more
europepmc +6 more sources
High-resolution structure determination using high-throughput electron cryo-tomography. [PDF]
Acta Crystallogr D Struct Biol, 2022 Tomographic reconstruction of frozen-hydrated specimens followed by extraction and averaging of sub-tomograms has successfully been used to determine the structure of macromolecules in their native environment at resolutions that are high enough to reveal molecular level interactions.
Liu HF, Zhou Y, Bartesaghi A.
europepmc +3 more sources
Electron cryo-tomography structure of axonemal doublet microtubule from Tetrahymena thermophila. [PDF]
Life Sci Alliance, 2022 Doublet microtubules (DMTs) provide a scaffold for axoneme assembly in motile cilia. Aside from α/β tubulins, the DMT comprises a large number of non-tubulin proteins in the luminal wall of DMTs, collectively named the microtubule inner proteins (MIPs).
Li S +4 more
europepmc +8 more sources
Electron cryo-tomography provides insight into procentriole architecture and assembly mechanism. [PDF]
Elife, 2019 Summary Centriole is an essential structure with multiple functions in cellular processes. Centriole biogenesis and homeostasis is tightly regulated. Using electron cryo-tomography (cryoET) we present the structure of procentrioles from Chlamydomonas reinhardtii .
Li S +3 more
europepmc +7 more sources
Electron cryo-tomography reveals the subcellular architecture of growing axons in human brain organoids. [PDF]
Elife, 2021 During brain development, axons must extend over great distances in a relatively short amount of time. How the subcellular architecture of the growing axon sustains the requirements for such rapid build-up of cellular constituents has remained elusive.
Hoffmann PC +6 more
europepmc +4 more sources