Results 51 to 60 of about 2,460,746 (279)
In Situ Studies of Mitochondrial Translation by Cryo-Electron Tomography
, 2021 Cryo-electron tomography (cryo-ET) enables the three-dimensional (3D) visualization of macromolecular complexes in their native environment (in situ).
Englmeier, Robert +3 more
core
napari plugins for cryo-electron tomography
, 2022 Presentation given at a workshop titled "computational frontiers in cryo-electron tomography", organised by the Chan Zuckerberg Initiative Slides were originally authored on google slides - pdf is static (no videos) and pptx may contain formatting ...
Burt, Alister
core +1 more source
Mind the Gap: How Some Viruses Infect Their Hosts
Viruses, 2010 Cryo-electron microscopy (Cryo-EM) and cryo-electron tomography (Cryo-ET) provide structural insights into complex biological processes. The podoviridae are dsDNA containing phage with short, non-contractile tails which nevertheless translocate their DNA
Peter E. Prevelige
doaj +1 more source
Current Research in Structural Biology, 2022 Studying bacterial cell envelope architecture with electron microscopy is challenging due to the poor preservation of microbial ultrastructure with traditional methods.
Danielle L. Sexton +3 more
doaj +1 more source
Electron Cryo-Tomography of Nanowires in Shewanella Oneidensis MR-1 [PDF]
Biophysical Journal, 2016 The dissimilatory metal-reducing bacterium Shewanella oneidensis MR-1 is known to make ‘nanowires,’ conductive cell appendages used in electron transport. Novel live fluorescence imaging techniques recently clarified that the conductive filaments in S. oneidensis are not pili, as previously thought, but rather extensions of the outer membrane. However,
Subramanian, Poorna +3 more
openaire +2 more sources
Biophysical approaches for studying viral entry
FEBS Letters, EarlyView.Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley +1 more source
FEBS Letters, EarlyView.Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav +2 more
wiley +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
Assembly and Imaging Set up of PIE-Scope
Bio-Protocol, 2020 Cryo-Electron Tomography (cryo-ET) is a method that enables resolving the structure of macromolecular complexes directly in the cellular environment. However, sample preparation for in situ Cryo-ET is labour-intensive and can require both cryo-lamella ...
Sergey Gorelick +4 more
doaj +1 more source
Three-dimensional structural analysis of eukaryotic flagella/cilia by electron cryo-tomography [PDF]
Journal of Synchrotron Radiation, 2010 ISSN:0909 ...
Bui Khanh Huy +2 more
openaire +5 more sources