Results 71 to 80 of about 2,460,746 (279)

Structural insights into muscle organisation by electron cryo-tomography

, 2022
Movement is the essence of life in the animal realm. Skeletal muscle is an essential tissue specialised for movement. Muscle cells are multi-nucleated cells containing bundles of myofibrils, which are segmented into the smallest contractile units, named sarcomeres.
openaire   +3 more sources

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Unraveling atomic complexity from frozen samples

Structural Dynamics
Cryo-electron microscopy (cryo-EM) is a significant driver of recent advances in structural biology. Cryo-EM is comprised of several distinct and complementary methods, which include single particle analysis, cryo-electron tomography, and microcrystal ...
Yasmeen N. Ruma, Brent L. Nannenga, Tamir Gonen   +2 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Improving adhesion and targeting of adherent areas for high-throughput cryo electron tomography of vitreous sections

Journal of Structural Biology: X
Cellular cryo electron tomography explores tissue and cells in their unstained flash-frozen native state, revealing in situ the structure of macromolecules together with their local environment and interactions with partners, also known as molecular ...
Fatima Taiki, Helmut Gnaegi, Diana Grewe, Nils Marechal, Mikhail Eltsov, Amélie Leforestier   +5 more
doaj   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

Fluorination: A strategy to tune the interactions of molecules and nanoscale systems with biological matter

FEBS Open Bio, EarlyView.
This minireview explores fluorination as a key strategy to optimize therapeutic molecules and nanocarriers. By incorporating different fluorinated motifs—from single atoms to linear and branched chains—drug delivery, cellular uptake, and colloidal stability can be improved and fine‐tuned.
Martina Beccalli, Beatrice Lucia Bona, Francesca Baldelli Bombelli   +2 more
wiley   +1 more source

Spatially Regulated Lithium Plating via Li2O‐Rich Interphase Enables Durable Fast‐Charging Hybrid Lithium Batteries

Advanced Functional Materials, EarlyView.
A Li2O‐rich solid electrolyte interphase (SEI) is engineered on graphite through a propylene carbonate‐based electrolyte to spatially regulate Li plating. The homogeneous interphase lowers Li nucleation barriers, promotes conformal Li deposition around graphite particles, and suppresses dendritic accumulation, enabling durable hybrid Li‐ion/Li‐metal ...
Robert Kuphal, Jingjing Liu, Junxiang Liu, Tao Ma, Yi Ding, Mahmood Haq, Satish Udpa, Stephen J. Harris, Chengcheng Fang   +8 more
wiley   +1 more source

Visualization of lysosomal membrane proteins by cryo electron tomography

Nature Communications
Lysosomes are essential organelles for cellular homeostasis and signaling, with dysfunction linked to neurological disorders, lysosomal storage diseases, and cancer. While proteomics has advanced our understanding of lysosomal composition, the structural
Bridget M. McVeigh, José J. De Jesús-Pérez, Dirk H. Siepe, Prerana Gogoi, Shrawan Kumar Mageswaran, Marian Kalocsay, Elaine M. Mihelc, Vera Y. Moiseenkova-Bell   +7 more
doaj   +1 more source