Results 81 to 90 of about 2,460,746 (279)

Correlative cryo-fluorescence light microscopy and cryo-electron tomography of streptomyces

, 2014
Light microscopy and electron microscopy are complementary techniques that in a correlative approach enable identification and targeting of fluorescently labeled structures in situ for three-dimensional imaging at nanometer resolution.
MullerReichert, T., Willemse, J., Verkade, P., Celler, K., Koning, R.I., Bos, E., Wezel, G.P. van, Koster, A.J.   +7 more
core   +1 more source

Contrast transfer function correction applied to cryo-electron tomography and sub-tomogram averaging [PDF]

, 2009
Cryo-electron tomography together with averaging of sub-tomograms containing identical particles can reveal the structure of proteins or protein complexes in their native environment.
Fuller, Stephen D., Briggs, John A. G., Giulia Zanetti, Riches, James D., Zanetti, Giulia, James D. Riches, Stephen D. Fuller, John A.G. Briggs, Briggs, J., Riches, J.D., Riches, J., Fuller, S.D., Fuller, S., Zanetti, G., Briggs, J.A.G.   +14 more
core   +1 more source

Cryo-ET data processing can be roughly divided into 3 blocks.

, 2021
Preprocessing (top) transforms raw experimental data, usually in the form of multiframe micrographs, into 3D images called tomograms. Particle picking (middle) derives particle position and orientation information from tomograms as well as an initial ...
Irina Gutsche (207422), Alister Burt (6242912), Tom Dendooven (3341570), Lorenzo Gaifas (11337787)   +3 more
core   +1 more source

Preferential Interfacial Engineering of Hydrogels via Interdigitated Nanoparticle Assembly

Advanced Functional Materials, EarlyView.
Robust surface functionalization of hydrogel surfaces is performed via interdigitated metal nanoparticle assembly, inducing strong affinity with the hydrogel matrix. The partially exposed nanoparticle domains act as interfacial nanobridges that mediate both chemical and physical bonding with diverse materials, including small molecules, fluorous ...
Hyeonjin Kim, Hyeseoung Lim, Ji Min Lee, Heeyeon Kang, Yongkwon Song, Woojae Chang, So‐Jin Park, Jin Yoo, Atsushi Hozumi, Jinhan Cho, Jongkuk Ko, Sanghyuk Wooh   +11 more
wiley   +1 more source

A Holistic Platform Approach for Developing Robust Pulmonary mRNA Delivery: Integrating Novel Phenolic Acid‐Derived Ionizable Lipids and Device Engineering

Advanced Functional Materials, EarlyView.
An integrated pulmonary mRNA delivery platform combining novel biodegradable syringic acid‐derived ionizable lipids, design‐of‐experiments formulation optimization, and vibrating‐mesh nebulizer engineering enabled stable aerosolization and efficient lung delivery.
Neha Kaushal, Younan Ma, Asad Khanmohammed, Dipen Parande, Hanming Zhang, Saket Dolare, Juliet Chen, Apiwat Wangweerawong, Saswata Karmakar, Hongfeng Deng, Frank Koppenhagen, Shraddha Sharma, Eric Reyes, Sherry He, Lianne Boeglin, Caroline Woo, Azadeh Paksa, Ayed Allawzi, Anusha Dias, Gregory Troiano, Shrirang Karve, Frank DeRosa   +21 more
wiley   +1 more source

Structural Conservation and Adaptation of the Bacterial Flagella Motor

Biomolecules, 2020
Many bacteria require flagella for the ability to move, survive, and cause infection. The flagellum is a complex nanomachine that has evolved to increase the fitness of each bacterium to diverse environments. Over several decades, molecular, biochemical,
Brittany L. Carroll, Jun Liu
doaj   +1 more source

Beyond Thin Film Limits—Superhard and Hydrogen‐Free Amorphous Carbon Coatings With Over 100 µm Thickness

Advanced Functional Materials, EarlyView.
For the first time, an extreme‐thick laser‐arc PVD ta‐C (DLC) coating exceeding 100 µm is demonstrated, opening up new possibilities for ultra‐high load bearing capacity. This superhard amorphous carbon architecture combines >50% sp3 bonding, robust adhesion and self‐supporting performance, shielding substrates from plastic flow under extreme contact ...
Martin Zawischa, Frank Kaulfuß, Stefan Makowski, Volker Weihnacht   +3 more
wiley   +1 more source

Extracellular filaments revealed by affinity capture cryogenic-electron tomography

Nature Communications
Cryogenic-electron tomography (cryo-ET) has provided an unprecedented glimpse into the nanoscale architecture of cells by combining cryogenic preservation of biological structures with electron tomography. Micropatterning of extracellular matrix proteins
Leeya Engel, Magda Zaoralová, Momei Zhou, Alexander R. Dunn, Stefan L. Oliver   +4 more
doaj   +1 more source

Dynamic Observation of Lacy Pattern Formation in a Bone Tissue Model Using Liquid Phase Scanning Transmission Electron Microscopy

Advanced Functional Materials, EarlyView.
Here, we establish a low‐dose liquid‐phase electron microscopy workflow for real‐time observation of biological processes in hydrated conditions. Beam damage is reduced by imaging only 20% of the pixels and reconstructing the complete image using an inpainting algorithm.
Luco Rutten, Avital Wagner, Rona Roverts, Luco Buise, Ben Joosten, Anat Akiva, Elena Macías‐Sánchez, Nico Sommerdijk   +7 more
wiley   +1 more source

Cryo-lift-out Technique for Cryo-electron Tomography of Tissue Samples

Shengwu huaxue yu shengwu wuli jinzhan
Cryo-electron tomography (cryo-ET) enables the determination of high-resolution three-dimensional structures of macromolecular complexes within cells in a near-physiological state, providing crucial structural insights into fundamental life processes ...
QIN Chang-Dong, GUO Qiang, GAO Ning
doaj   +1 more source