Results 91 to 100 of about 2,460,746 (279)

A cylindrical specimen holder for electron cryo-tomography

, 2013
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openaire   +1 more source

In Situ Interfacial Assembly of Dynamic Semi‐Interpenetrating Biopolymer Networks for Robust and Mechanically Adaptive Droplet Interfaces

Advanced Functional Materials, EarlyView.
A dynamic interfacial semi‐interpenetrating polymer network is constructed by sequential assembly of β‐sheet‐rich SF and CS‐RL. The SF framework provides mechanical strength, while dynamically associated CS‐RL enables local rearrangement, allowing droplets to rapidly reconfigure without loss of interfacial integrity.
Yaping Zhang, Yujie Liu, Yikai Xu, Xunda Feng, Ling Zhang, Hongbo Zeng, Hao Liu, Xiaohui Mao   +7 more
wiley   +1 more source

Fast and accurate defocus modulation for improved tunability of cryo-EM experiments

IUCrJ, 2020
Current data collection strategies in electron cryo-microscopy (cryo-EM) record multiframe movies with static optical settings. This limits the number of adjustable parameters that can be used to optimize the experiment.
Radostin Danev, Hirofumi Iijima, Mizuki Matsuzaki, Sohei Motoki   +3 more
doaj   +1 more source

Developments in cryo-electron tomography for in situ structural analysis

, 2015
Structural analysis of macromolecular assemblies and their remodeling during physiological processes is instrumental to defining the fundament of cellular and molecular biology.
Sorrentino, Simona, Medalia, Ohad, Harapin, Jan, Sapra, K Tanuj, Dubrovsky, Anna, Dubrovsky A, Sapra KT, Medalia O, Harapin J, Sorrentino S   +9 more
core   +1 more source

Ionic Gating of Liquid‐Crystal‐Like Pteridine Assemblies Enables Tunable Light Scattering

Advanced Materials, EarlyView.
Tunable optical properties emerge through developmentally regulated ionic gating, which transforms disordered, ultraviolet (UV)‐absorbing organelles into concentrically ordered, liquid‐crystal‐like light scatterers. By integrating analyses of pteridine composition, potassium‐dependent assembly, hierarchical ultrastructure, and optical behavior, the ...
Sourabh Bera, Zohar Eyal, Yael Noy, Yuval Barzilay, Siddharth Sahoo, Srikant Moharana, Avi Baram, Tali Lerer‐Goldshtein, Neta Varsano, Bar Bader, Lothar Houben, Uwe Heinig, Iddo Pinkas, Moshe Goldsmith, Tamar Unger, Shira Albeck, Andrea Sorrentino, Maxim Itkin, Sergey Malitsky, Ziv Porat, Ido Hadar, Venkata Jayasurya Yallapragada, Dvir Gur   +22 more
wiley   +1 more source

Cryo-electron tomography pipeline for plasma membranes

Nature Communications
Cryo-electron tomography (cryoET) provides sub-nanometer protein structure within the dense cellular environment. Existing sample preparation methods are insufficient at accessing the plasma membrane and its associated proteins.
Willy W. Sun, Dennis J. Michalak, Kem A. Sochacki, Prasanthi Kunamaneni, Marco A. Alfonzo-Méndez, Andreas M. Arnold, Marie-Paule Strub, Jenny E. Hinshaw, Justin W. Taraska   +8 more
doaj   +1 more source

Purification and Cryo-Electron Microscopy Analysis of Bacterial Appendages

Bio-Protocol
A number of extracellular helical protein polymers are crucial for supporting bacterial motility. The bacterial flagellum is a polymeric appendage used to support cellular motility.
Juan Sanchez, Joseph Baumgardt, Elizabeth Wright   +2 more
doaj   +1 more source

Immersion Freezing of Cell Monolayers for Cryo-Electron Tomography

, 2011
INTRODUCTIONCryo-transmission electron microscopy (cryo-TEM) is not limited to the visualization of suspended macromolecules in their native hydrated state. It can also be used to elucidate the molecular architecture of peripheral parts of adherent cells:
Marlene Brandstetter, Veronika I. Wonesch, Guenter P. Resch, Edit Urban   +3 more
core   +1 more source

Towards Temporal Resolution in Correlative Cryo-electron Tomography

, 2023
Cryo-electron tomography (cryo-ET) has given unprecedented insights into the structure of macromolecules in their native cellular environment. This technique bridges the gap between high-resolution ex-situ single particle analysis and low-resolution, in ...
Brenner, J. ; https://orcid.org/, Plitzko, J., Wilfling, F. ; https://orcid.org/, Klumpe, S.   +3 more
core   +1 more source

Differential Sedimentation and Cryo‐TEM Mapping Resolve Structural Heterogeneity in Conjugated Polymer Nanowires for Programmable Stretchable Electronics

Advanced Materials, EarlyView.
Density‐driven differential sedimentation fractionates solution‐assembled P3HT nanowires into crystalline and disordered constituents. High‐resolution cryo‐TEM directly visualizes the molecular chain packing within individual nanowires in real space, and recombining the fractions programs the mobility–stretchability trade‐off without chemical ...
Junyeon Yoon, Jun Ho Hwang, Eunji Lee
wiley   +1 more source