Results 101 to 110 of about 2,460,746 (279)

Frontiers in integrative structural modeling of macromolecular assemblies

QRB Discovery
Integrative modeling enables structure determination for large macromolecular assemblies by combining data from multiple experiments with theoretical and computational predictions.
Kartik Majila, Shreyas Arvindekar, Muskaan Jindal, Shruthi Viswanath   +3 more
doaj   +1 more source

Cryo-electron tomography for the structural study of mitochondrial translation

, 2019
Cryo-electron tomography (cryo-ET) enables the three-dimensional (3D) structural characterization of macromolecular complexes in their physiological environment.
Englmeier, Robert, Förster, Friedrich, Cryo-EM, Sub Cryo - EM   +3 more
core  

Cryo-scanning transmission electron tomography of vitrified cells

, 2014
Cryo-electron tomography (CET) of fully hydrated, vitrified biological specimens has emerged as a vital tool for biological research. For cellular studies, the conventional imaging modality of transmission electron microscopy places stringent constraints
Michael Elbaum, Houben, Lothar, Elbaum, Michael, Wolf, Sharon Grayer, Sharon Grayer Wolf, Lothar Houben   +5 more
core   +1 more source

Electron Tomography and Correlative Approaches in Platelet Studies [PDF]

, 2018
Blood platelets play a central role in the arrest of bleeding and the development of thrombosis. Unraveling the complex processes of platelet biogenesis from megakaryocytes, platelet adhesion, aggregation, and secretory responses are important topics in ...
Geerts, Willie J C, Kasia B. Engberts, Heijnen, Harry F G, Willie J. C. Geerts, Seinen, Cor, Engberts, Kasia B, Harry F. G. Heijnen, Cor Seinen, Crystal and Structural Chemistry, Cryo-EM, Sub Cryo - EM   +10 more
core   +1 more source

Increasing Lipid Nanoparticle mRNA Copy Number Improves Transfection Potency

Advanced Materials, EarlyView.
As lipid nanoparticle (LNP) systems are the leading platform for in vivo nucleic acid delivery, improving transfection potency of these systems is of considerable interest. In this work, we explored a post‐mixing formulation method involving a two‐step buffer exchange that increased the number of mRNA copies within each LNP while reducing the number of
Yao Zhang, Jerry Leung, Judy Cheng, Arash Momeni, Albert Kamanzi, Ariadne Tuckmantel Bido, Suiyang Liao, Emma Kang, Colton Strong, Christian J. Kastrup, Sabrina Leslie, Miffy Hok Yan Cheng, Pieter R. Cullis   +12 more
wiley   +1 more source

Purification of Herpesvirus Virions and Capsids

Bio-Protocol, 2014
This protocol was designed for large-scale purification of herpesvirus particles by cell culture. Virions and capsids are isolated from extracellular culture media and cell nuclei, respectively.
Xinghong Dai, Z. Zhou
doaj   +1 more source

Cryo-ET tilt series for the 3D structure presented in the figures of the paper entitled "Angle Between DNA Linker and Nucleosome Core Particle Regulates Array Compaction by Individual-Particle Cryo-Electron Tomography"

Cryo-ET raw images and tilt series used for the published 3D structures shown in the figures of the paper entitled "Angle Between DNA Linker and Nucleosome Core Particle Regulates Array Compaction by Individual-Particle Cryo-Electron Tomography"
Jianfang Liu (13988585), P.D. Ashby (7343498), Jinhui Tao (18418375), Gang Ren (18417882), Carlos Bustamante (18418381), César-Díaz Celis (13988572), Meng Zhang (13988567)   +6 more
core   +1 more source

Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography

, 2014
Cryo-electron tomography (CET) produces three-dimensional images of cells in a near-native state at macromolecular resolution, but identifying structures of interest can be challenging. Here we describe a correlated cryo-PALM (photoactivated localization
Songye Chen, Jensen, G., Treuner-Lange, A., Anke Treuner-Lange, Yi-Wei Chang, Chang, Y., Elitza I Tocheva, Stephanie Löbach, Grant J Jensen, Chen, S., Sogaard-Andersen, L., Lotte Søgaard-Andersen, Tocheva, E., Loebach, S.   +13 more
core   +1 more source

Charge‐Reversed Lipid Nanoparticles Enable Encapsulation of Pt2L4 Nanocages for In vivo Anti‐Cancer Treatment

Advanced Materials, EarlyView.
Charge‐reversed lipid nanoparticles (revLNPs) enable efficient encapsulation and delivery of cationic Pt2L4 nanocages. Encapsulation preserves potent anticancer activity while improving circulation, reducing vascular leakage, and promoting favorable biodistribution in vivo.
Renzo A. Knol, Bochuan Hu, Eduard O. Bobylev, Martina Farber, Petrus C. M. Laan, Thomas J. F. Kock, Bauke Fontein, Johanna Erbani, Oscar Escalona‐Rayo, Seline S. Roose, Tom Keijer, Gabriel Forn‐Cuní, B. Ewa Snaar‐Jagalska, Leila Akkari, Joost N. H. Reek, Alexander Kros, Dennis Aschmann   +16 more
wiley   +1 more source

CEMOVIS; cryo-electron microscopy of vitreous sections

, 2008
Institut für AnatomieNotes: 2008016244 GBA7A2002 edited by Annie Cavalier, Danièle Spehner, Bruno M. Humbel. ill. ; 26 cm. Includes bibliographical references and index. Slam-freezing, metal-mirror freezing / Danièle Spehner and Ludwig Edelmann -- Plunge-
Al-Amoudi, A., Dubochet, J., Bouchet-Marquis, C., Zuber, Benoît, Eltsov, M.   +4 more
core