Results 31 to 40 of about 14,888,742 (175)
In Situ Studies of Mitochondrial Translation by Cryo-Electron Tomography
, 2021 Cryo-electron tomography (cryo-ET) enables the three-dimensional (3D) visualization of macromolecular complexes in their native environment (in situ).
Englmeier, Robert +3 more
core
Cryo-EM structural determination of PfFNT.
, 2021 (A) A representative cryo-EM micrograph of PfFNT. (B) A representative 2D classification average. (C) Flowchart for EM data processing of PfFNT datasets. Details can be found in the Methods. cryo-EM, cryogenic-electron microscopy; EM, electron microscopy;
Yanxia Zhou (1447465) +17 more
core +1 more source
FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles
eLife, 2019 The fibroblast growth factor FGF21 was labeled with molecularly defined gold nanoparticles (AuNPs), applied to human adipocytes, and imaged by cryo-electron tomography (cryo-ET). Most AuNPs were in pairs about 80 Å apart, on the outer cell surface. Pairs
Maia Azubel +6 more
doaj +1 more source
Cryo-EM data, refinement, and validations.
, 2022 Cryo-EM data, refinement, and validations.
Gerald S. Baron (300505) +10 more
core +1 more source
eLife, 2021 During brain development, axons must extend over great distances in a relatively short amount of time. How the subcellular architecture of the growing axon sustains the requirements for such rapid build-up of cellular constituents has remained elusive ...
Patrick C Hoffmann +6 more
doaj +1 more source
Engineering Microbial Particles for Next‐Generation Biomedical Platforms
Advanced Science, EarlyView.Microbe‐derived particles (MDPs), which include extracellular vesicles, outer membrane vesicles, inclusion bodies, polysaccharide particles, and virus‐like particles, represent a rapidly expanding category of bioinspired nanomaterials. With their natural origin, intrinsic biocompatibility, and highly programmable functionality, MDPs serve as a ...
Yuting Li +7 more
wiley +1 more source
Assembly and Imaging Set up of PIE-Scope
Bio-Protocol, 2020 Cryo-Electron Tomography (cryo-ET) is a method that enables resolving the structure of macromolecular complexes directly in the cellular environment. However, sample preparation for in situ Cryo-ET is labour-intensive and can require both cryo-lamella ...
Sergey Gorelick +4 more
doaj +1 more source
Advanced Science, EarlyView.This Review shows how cerium oxide helps water‐splitting catalysts work better and last longer. It explains how CeO2 tunes electronic structure, oxygen‐vacancy behavior, and surface reconstruction, while adapting to alkaline, acidic, and seawater conditions.
Zhen Cui +9 more
wiley +1 more source
Advanced cryo-tomography workflow developments - correlative microscopy, milling automation and cryo-lift-out [PDF]
, 2020 Cryo-electron tomography (cryo-ET) is a groundbreaking technology for 3D visualisation and analysis of biomolecules in the context of cellular structures. It allows structural investigations of single proteins as well as their spatial arrangements within
Erdmann, P. +9 more
core +1 more source
STAR Protocols, 2022 Summary: We recently demonstrated how lipid droplets can serve as in situ fiducials for correlating cryo-fluorescence microscopy (cryo-FM) and cryo-focused ion beam scanning electron microscopy (cryo-FIB-SEM) datasets of mammalian cells grown on grids ...
Nadav Scher +3 more
doaj +1 more source