Results 41 to 50 of about 14,888,742 (175)

Phosphonium Ionic Liquids for Electrochemical Energy Storage: Structure–Property–Interphase Relationships and Design Rule

Advanced Science, EarlyView.
Phosphonium ionic liquids offer a versatile platform for safer and more durable energy storage. This Review connects cation–anion structure with solvation, ion transport, electrochemical stability, and interphase formation across diverse battery chemistries.
Amir Haghipour, Ishanka Thennakoon, Aishwaryaba Jadeja, Daniel Rauber, Markus Gallei, Rolf Hempelmann, Sangwon Kim, Jungtae Kim   +7 more
wiley   +1 more source

Classification in cryo-electron tomograms [PDF]

, 2019
Different imaging techniques allow us to study the organization of life at different scales. Cryo-electron tomography (cryo-ET) has the ability to three-dimensionally visualize the cellular architecture as well as the structural details of macro ...
Multimedia, Förster, F.G., van der Schot, G., Xu, Min, Sub Multimedia, Du, X, Zeng, X, Kervrann, Charles, Himes, Benjamin A., Moebel, Emmanuel, Veltkamp, R.C., Cryo-EM, Gubins, I., Martinez-Sanchez, Antonio, Chang, L, Lai, T, Zhu, Z, Sub Cryo - EM   +17 more
core   +1 more source

Cryo-electron microscopy in the fight against COVID-19—mechanism of virus entry

Frontiers in Molecular Biosciences, 2023
Cryogenic electron microscopy (cryo-EM) and electron tomography (cryo-ET) have become a critical tool for studying viral particles. Cryo-EM has enhanced our understanding of viral assembly and replication processes at a molecular resolution.
Satish Bodakuntla, Christopher Cyrus Kuhn, Christian Biertümpfel, Naoko Mizuno, Naoko Mizuno   +4 more
doaj   +1 more source

Automated Bacterial Identification and Morphological Feature Analysis in Low‐Dose Cryo‐EM Using YOLOv11

Advanced Intelligent Discovery, EarlyView.
AI‐based tools enable rapid characterization of bacterial ultrastructure in low‐dose cryogenic transmission electron microscopy. The envelope thickness tool quantifies membrane thickness and anisotropy. The flagella module analyzes filament morphology and detects cell‐flagella contacts.
Sita Sirisha Madugula, Lynnicia N. Massenburg, Spenser R. Brown, Amber N. Bible, Chanda R. Harris, Lance X. Zhang, Kiara Parker, Scott T. Retterer, Jennifer L. Morrell‐Falvey, Rama K. Vasudevan, Alexis N. Williams   +10 more
wiley   +1 more source

Cryo-CLXEM introduces cryo-SXT to bridge the resolution gap in cryo-CLEM

Communications Biology
Cryo-imaging in cellular biology provides the means to visualize the cellular interior at close-to-native conditions. A cornerstone in the field has been cryo-correlative light and electron microscopy (cryo-CLEM), with cryo-visible light fluorescent ...
Johannes Groen, Anastasia Gazi, Sergey Kapishnikov, Anne Brelot, Matthijn Vos, Jost Enninga, Eva Pereiro, Anna Sartori-Rupp   +7 more
doaj   +1 more source

Labeling and Localization Strategies for In Situ Cryo-Electron Tomography Across the Viral Life Cycle

Viruses
Cryo-electron tomography (Cryo-ET) has emerged as a transformative tool for visualizing viral components within their native cellular environment, enabling structural interrogation of viral life cycle events at nanometer resolution without chemical ...
Yoon Ho Park, Rana Kim, Kun-Ho Song, Hyun Suk Jung   +3 more
doaj   +1 more source

Cryo‐EM Analysis of Adenovirus Vector Reveals Purification Conditions Leading to Loss of Viral Fiber Proteins and Reduced Infectivity

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Vaccines and gene therapies require efficient delivery of genetic material to host cells. Viral vectors leverage viruses' ability to enter host cells to achieve this. Following the SARS‐CoV‐2 vaccine and novel gene therapies that utilize adenovirus vectors, there has been an increase in the demand for large‐scale production of vectors.
Betsy Widdicombe, Joseph D. Barritt, Nichakorn Pipatpadungsin, Braulio Carrillo Sanchez, Dale Stibbs, Lisa Cooper, Sarah L. Rouse, Daniel G. Bracewell   +7 more
wiley   +1 more source

Isolation, cryo-laser scanning confocal microscope imaging and cryo-FIB milling of mouse glutamatergic synaptosomes.

PLoS ONE, 2022
Ionotropic glutamate receptors (iGluRs) at postsynaptic terminals mediate the majority of fast excitatory neurotransmission in response to release of glutamate from the presynaptic terminal.
Prerana Gogoi, Momoko Shiozaki, Eric Gouaux   +2 more
doaj   +1 more source

Amyloid-β oligomers, curvilinear and annular assemblies, imaged by cryo-ET, cryo-EM, and AFM. [PDF]

Sci Adv
Prefibrillar structures of the amyloid-β (Aβ) peptide are central to cytotoxicity in Alzheimer's disease. Time-resolved imaging of oligomers has enabled quantification of their extension. A snapshot of these prefibrillar assemblies has been characterized
Liang R, Khursheed A, Tahirbegi B, Torres-Flores AP, Qi S, Tian Y, Zhang H, Szwedziak P, Volkov VA, Darbari VC, Viles JH.   +10 more
europepmc   +2 more sources

Investigating the Cytoskeleton of DRGs Using Cryo‐Electron Microscopy and Deep Learning

Cytoskeleton, EarlyView.
ABSTRACT We describe a method for determining the ultrastructural organization of axons and varicosities of cultured dorsal root ganglion (DRG) neurons using cryogenic electron microscopy (cryo‐EM). Cryo‐EM reveals the dimensions, proximity, and overall organization of biological specimens in a near‐native state, avoiding artifacts of fixation and ...
M. Neal Waxham, Mihir Relan, Matthew T. Swulius, Andrea K. H. Stavoe   +3 more
wiley   +1 more source