Results 61 to 70 of about 14,888,742 (175)

Structural Polymorphism of polyG Inclusions Revealed by In Situ Cryo‐Electron Tomography

Advanced Science, Volume 13, Issue 56, 9 October 2026.
Correlative cryo‐electron tomography in primary cortical neurons and NIID mouse brain tissue reveals that polyG inclusions are interconnected ribbon‐like assemblies rather than canonical amyloid fibrils. Multiple compartment‐specific ribbon states show distinct 26S proteasome accessibility, while cytoplasmic ribbons contact and deform ER‐like ...
Yunwen Qian, Yalan Wan, Chen Chen, Xiaoyu Zheng, Wenjing Du, Junhan Yang, Zhihao Quan, Biyu Yang, Jiaxi Yu, Jie Zheng, Zhaoxia Wang, Jianwen Deng, Qiang Guo   +12 more
wiley   +1 more source

Cryo-EM data collection, processing, and validation.

, 2023
Cryo-EM data collection, processing, and validation.
Anila Yasmeen (460713), Philippe Colin (128052), Thomas Ketas (164120), Max Crispin (346213), Aleksandar Antanasijevic (475614), P. J. Klasse (15944555), John P. Moore (260880), Joel D. Allen (5285486), Gemma E. Seabright (5285489), Kimmo Rantalainen (6954842), Rajesh P. Ringe (3118764), Andrew B. Ward (7832180), Jeong Hyun Lee (7826723), Gabriel Ozorowski (711509)   +13 more
core   +1 more source

The resolution revolution revisited

IUCrJ
In this issue of IUCrJ, Subramaniam, Kühlbrandt & Henderson present an overview of the remarkable progress that has been made in electron cryo-microscopy and electron cryo-tomography.
Edward H. Egelman
doaj   +1 more source

PolyG Fibrils Coalesce Into Nuclear Ribbons That Engage Proteostasis Machinery in Neuronal Intranuclear Inclusion Disease

Advanced Science, Volume 13, Issue 56, 9 October 2026.
In NIID, expanded NOTCH2NLC repeats give rise to nuclear polyG inclusions. Tracer‐guided in situ cryo‐electron tomography enables cross‐scale structural analysis from mouse brain to native neuronal nuclei, revealing dense‐core/peripheral‐halo inclusions built from compact polyG ribbons.
Hui Dong, Yongcheng Pan, Zhiyao Tang, Yuxuan Yao, Guicong Zhang, Junpu Wang, Haonan Xiao, Yun Tian, Beisha Tang, Dan Li, Qiang Guo, Ruijun Tian, Qiong Liu, Cong Liu   +13 more
wiley   +1 more source

Progress in the development of an ultrafast pulsed ponderomotive phase plate for cryo-electron tomography

Structural Dynamics
Cryo-electron tomography (cryo-ET) is a powerful modality for resolving cellular structures in their native state. While single-particle cryo-electron microscopy excels in determining protein structures purified from recombinant or endogenous sources due
Daniel X. Du, Adam C. Bartnik, Cameron J. R. Duncan, Usama Choudhry, Tanya Tabachnik, Chaim Sallah, Ebrahim Najafi, Ding-Shyue Yang, Jared M. Maxson, Anthony W. P. Fitzpatrick   +9 more
doaj   +1 more source

Phase Separation, Material State, and Condensate Fate in Mammalian Autophagy

Advanced Science, Volume 13, Issue 54, 28 September 2026.
ABSTRACT Biomolecular phase separation has emerged as a key organizing principle in macroautophagy (hereafter autophagy). In mammalian cells, phase‐separated condensates not only serve as substrates for selective degradation, but also act as dynamic platforms for cargo recognition, signaling integration, and autophagosome assembly.
Yuanqiang Lin, Mingran Dai, Enyong Dai, Rui Kang, Daniel J. Klionsky, Daolin Tang, Yu Sun, Ningning Lv, Junnan Jiang   +8 more
wiley   +1 more source

Cryo-ET images in front and back of migrating cells

Cryo-ET segmentation and 3D rendering of ER and PM from the front or back of cells migrating on stripes.
Bo Gong (18390543)
core   +1 more source

Correlative voltage imaging and cryo-electron tomography bridge neuronal activity and molecular structure

Nature Communications
Neurons exhibit varying electrophysiological properties due to dynamic changes in spatiotemporal molecular networks. In situ cryo-electron tomography (cryo-ET) provides advantages for high-resolution visualization of macromolecular complexes within their
Mingyu Jung, Gwanho Ko, Dongsung Lim, Seonghoon Kim, Sojeong Kim, Young-Joon Kim, Myunghwan Choi, Soung-Hun Roh   +7 more
doaj   +1 more source

Graph identification of proteins in tomograms (GRIP‐Tomo) 2.0: Topologically aware classification for proteins

Protein Science, Volume 35, Issue 9, September 2026.
Abstract Cryo‐electron tomography (cryo‐ET) enables structural characterization of biomolecules under near‐native conditions. Existing approaches for interpreting the resulting three‐dimensional volumes are computationally expensive and have difficulty interpreting density associated with small proteins/complexes.
Chengxuan Li, August George, Reece Neff, Doo Nam Kim, Trevor Moser, Kate Baldwin, Malio Nelson, Arsam Firoozfar, James E. Evans, Margaret S. Cheung   +9 more
wiley   +1 more source

Cryo-EM analysis of MmpL3-TMM III.

, 2021
(a) Data processing flow chart with particle distributions. The black box indicates the particle class used for further refinement. (b) Representative 2D classes. (c) FSC curves.
Christopher E. Morgan (11269227), Philip A. Klenotic (11269221), Meinan Lyu (11269224), Edward W. Yu (9745721), Chih-Chia Su (573213), Meng Cui (29943)   +5 more
core   +1 more source