Results 31 to 40 of about 12,575 (237)

Correlative Light and Electron Microscopy in Parasite Research

, 2012
The interaction of a parasite and a host cell is a complex process, which involves several steps: (1) attachment to the plasma membrane, (2) entry inside the host cell, and (3) hijacking of the metabolism of the host.
Loussert, C., Forestier, C.L., Humbel, B.M., Claire-Lise Forestier, Bruno M. Humbel, Loussert C, Humbel BM, Forestier CL, Céline Loussert   +8 more
core   +1 more source

Correlative light and immuno-electron microscopy of retinal tissue cryostat sections.

PLoS ONE, 2018
Correlative light-electron microscopy (CLEM) is a powerful technique allowing localisation of specific macromolecules within fluorescence microscopy (FM) images to be mapped onto corresponding high-resolution electron microscopy (EM) images.
Thomas Burgoyne, Amelia Lane, William E Laughlin, Michael E Cheetham, Clare E Futter   +4 more
doaj   +1 more source

Trafficking of Annexins during Membrane Repair in Human Skeletal Muscle Cells

Membranes, 2022
Defects in membrane repair contribute to the development of muscular dystrophies, such as Miyoshi muscular dystrophy 1, limb girdle muscular dystrophy (LGMD), type R2 or R12.
Coralie Croissant, Céline Gounou, Flora Bouvet, Sisareuth Tan, Anthony Bouter   +4 more
doaj   +1 more source

Serial-section Electron Tomography and Quantitative Analysis of Microtubule Organization in 3D-reconstructed Mitotic Spindles

Bio-Protocol, 2023
For the analysis of cellular architecture during mitosis, nanometer resolution is needed to visualize the organization of microtubules in spindles. Here, we present a detailed protocol that can be used to produce 3D reconstructions of whole mitotic ...
Robert Kiewisz, Daniel Baum, Thomas Müller-Reichert, Gunar Fabig   +3 more
doaj   +1 more source

Trimming the resolution gap in the study of molecular and cellular events by means of High Data Output and automated three-dimensional Correlative Light-Electron Microscopy approach

, 2009
Correlative light/electron microscopy (CLEM) allows the simultaneous observation of a given subcellular structure by fluorescence light microscopy (FLM) and electron microscopy.
Santangelo, C, Bianchini, P, Boccacci, P, Cortese, K, Tacchetti, C, Vicidomini, G, Diaspro, A, Gagliani, MC   +7 more
core   +1 more source

Correlative Organelle Microscopy: Fluorescence Guided Volume Electron Microscopy of Intracellular Processes

Frontiers in Cell and Developmental Biology, 2022
Intracellular processes depend on a strict spatial and temporal organization of proteins and organelles. Therefore, directly linking molecular to nanoscale ultrastructural information is crucial in understanding cellular physiology.
Sergey V. Loginov, Job Fermie, Job Fermie, Jantina Fokkema, Alexandra V. Agronskaia, Cilia De Heus, Gerhard A. Blab, Judith Klumperman, Hans C. Gerritsen, Nalan Liv   +9 more
doaj   +1 more source

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

FEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell, Thomas Walther, Egbert Hoiczyk   +2 more
wiley   +1 more source

Identification of a stereotypic molecular arrangement of endogenous glycine receptors at spinal cord synapses

eLife, 2021
Precise quantitative information about the molecular architecture of synapses is essential to understanding the functional specificity and downstream signaling processes at specific populations of synapses.
Stephanie A Maynard, Philippe Rostaing, Natascha Schaefer, Olivier Gemin, Adrien Candat, Andréa Dumoulin, Carmen Villmann, Antoine Triller, Christian G Specht   +8 more
doaj   +1 more source

Invisible but not inaccessible—Revealing transient oligomers formed by intrinsically disordered proteins with solution NMR and complementary methods

FEBS Letters, EarlyView.
Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley   +1 more source

Microsecond melting and revitrification of cryo samples with a correlative light-electron microscopy approach

Frontiers in Molecular Biosciences, 2022
We have recently introduced a novel approach to time-resolved cryo-electron microscopy (cryo-EM) that affords microsecond time resolution. It involves melting a cryo sample with a laser beam to allow dynamics of the embedded particles to occur.
Gabriele Bongiovanni, Oliver F. Harder, Marcel Drabbels, Ulrich J. Lorenz   +3 more
doaj   +1 more source