Results 51 to 60 of about 12,575 (237)

Effective investigation of murine femoral bone development utilizing correlative light and electron microscopy (CLEM)

Journal of Analytical Science and Technology, 2023
Background For effective investigation of the developing structure and chemistry of bone, comprehensive studies including compositional analysis can be achieved through the gradual observation from the micro- to nanometer scale via correlative light and ...
Yong-Eun Kwon, Jung-Kyun Kim, Youn-Joong Kim, A. Reum Je, Hwanuk Guim, Jin-Gyu Kim   +5 more
doaj   +1 more source

Call to action to properly utilize electron microscopy to measure organelles to monitor disease

European Journal of Cell Biology, 2023
This review provides an overview of the current methods for quantifying mitochondrial ultrastructure, including cristae morphology, mitochondrial contact sites, and recycling machinery and a guide to utilizing electron microscopy to effectively measure ...
Kit Neikirk, Edgar-Garza Lopez, Andrea G. Marshall, Ahmad Alghanem, Evan Krystofiak, Bartosz Kula, Nathan Smith, Jianqiang Shao, Prasanna Katti, Antentor Hinton, Jr.   +9 more
doaj   +1 more source

Density Measurement of Irradiated and Nonirradiated Zirconium Oxide by Ptychographic X‐Ray Computed Tomography (PXCT)

Advanced Engineering Materials, EarlyView.
Mass density governs the protective behavior of oxide layers on nuclear fuel cladding. Ptychographic X‐ray computed tomography measures zirconium oxide grown on Zircaloy‐4 under reactor and autoclave conditions, revealing nearly identical bulk densities of 5.1 ± 0.80 g cm−3.
Okan Yetik, Liliana I. Duarte, Malgorzata G. Makowska, Roland Brütsch, Andrej Bullemer, Ana Diaz, Mirko Holler, Andreas Menzel, Johannes Bertsch, Pavel Trtik   +9 more
wiley   +1 more source

Correlative Light and Electron Microscopy Reveals the HAS3-Induced Dorsal Plasma Membrane Ruffles

International Journal of Cell Biology, 2015
Hyaluronan is a linear sugar polymer synthesized by three isoforms of hyaluronan synthases (HAS1, 2, and 3) that forms a hydrated scaffold around cells and is an essential component of the extracellular matrix.
Kirsi Rilla, Arto Koistinen
doaj   +1 more source

Inverse Identification of Energy‐Dependent Laser Absorptivity in NiTi Laser Powder‐Bed Fusion via Calibrated Melt Pool Simulation

Advanced Engineering Materials, EarlyView.
A combined experimental–computational framework identifies energy‐dependent laser absorptivity for NiTi in laser powder‐bed fusion, applicable to conduction and transition modes. Single‐track experiments and thermofluid smoothed particle hydrodynamics simulations are coupled through inverse analysis of melt pool geometry.
Mohamadreza Afrasiabi, Hadi Moutablaleh, Ignatius A. Setiawan, Mehrshad Mehrpouya   +3 more
wiley   +1 more source

A practical protocol for correlative confocal fluorescence and transmission electron microscopy characterization of extracellular vesicles

Microbiology Spectrum
Extracellular vesicles (EVs) have gained considerable attention in fungal biology research. However, imaging these membrane-bound particles presents challenges due to their nanoscopic size (typically
Juan M. Martínez-Andrade, Daniel Salgado-Bautista, Kendra Ramirez-Acosta, Ruben Darío Cadena-Nava, Meritxell Riquelme   +4 more
doaj   +1 more source

ultraLM and miniLM: Locator tools for smart tracking of fluorescent cells in correlative light and electron microscopy [version 1; referees: 2 approved, 1 approved with reservations]

Wellcome Open Research, 2016
In-resin fluorescence (IRF) protocols preserve fluorescent proteins in resin-embedded cells and tissues for correlative light and electron microscopy, aiding interpretation of macromolecular function within the complex cellular landscape.
Elisabeth Brama, Christopher J. Peddie, Gary Wilkes, Yan Gu, Lucy M. Collinson, Martin L. Jones   +5 more
doaj   +1 more source

Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes [PDF]

, 2020
Three-dimensional correlative light and electron microscopy (3D CLEM) is attaining popularity as a potential technique to explore the functional aspects of a cell together with high-resolution ultrastructural details across the cell volume.
Peurla , Markus, Sahlgren ,Cecilia, Xavier Heiligenstein, Jessica M. Rosenholm, Belevich , Ilya, Heiligenstein, Xavier, Rosenholm, Jessica M., Rosenholm, Jessica, Sahlgren, Cecilia, Cecilia Sahlgren, Ilya Belevich, Heiligenstein , Xavier, Huan-Cheng Chang, Prabhakar, Neeraj, Jokitalo, Eija, Eija Jokitalo, Peurla, Markus, Chang, Huan-Cheng, Belevich, Ilya, Markus Peurla, Neeraj Prabhakar, Heiligenstein Xavier, Peurla Markus, Belevich Ilya, Jokitalo Eija, Chang Huan-Cheng, Sahlgren Cecilia, Prabhakar Neeraj, Rosenholm Jessica M   +28 more
core   +1 more source

Influence of Scan Strategies in Electron Beam Powder Bed Fusion on Solidification, Microstructure, and High‐Temperature Compressive Properties of γ′‐Strengthened Inconel 738LC

Advanced Engineering Materials, EarlyView.
Experiments and thermophysical simulations were conducted to investigate the electron beam powder bed fusion electron beam (PBF‐EB/M) process for the γ′‐strengthened nickel‐based superalloy Inconel 738LC. The results demonstrate the impact of process‐induced microstructural variations on high‐temperature mechanical behavior, providing a basis for ...
Jan Niklas Petenati, Karthik Kumar Banku, Prasanth Bondi, Ivan Zhirnov, Yangyiwei Yang, Gordon Schmidt, Jürgen Christen, Georg Hasemann, Manja Krüger, Bai‐Xiang Xu, Bilal Gökce, Silja‐Katharina Rittinghaus   +11 more
wiley   +1 more source

Correlative Scanning Electron and Super-Resolution Structured Illumination Microscopy

Photonics
Correlative microscopy techniques are used for many different applications in the biological sciences because the comparison of different imaging methods allows researchers to gain more and often complementary information about their samples. Correlative
Joseph R. Hamilton, Summer K. Levis, Guy M. Hagen   +2 more
doaj   +1 more source