Correlative Organelle Microscopy: Fluorescence Guided Volume Electron Microscopy of Intracellular Processes [PDF]
Frontiers in Cell and Developmental Biology, 2022 Intracellular processes depend on a strict spatial and temporal organization of proteins and organelles. Therefore, directly linking molecular to nanoscale ultrastructural information is crucial in understanding cellular physiology.
Sergey V. Loginov +9 more
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A versatile enhanced freeze-substitution protocol for volume electron microscopy [PDF]
Frontiers in Cell and Developmental Biology, 2022 Volume electron microscopy, a powerful approach to generate large three-dimensional cell and tissue volumes at electron microscopy resolutions, is rapidly becoming a routine tool for understanding fundamental and applied biological questions.
Sébastien Bélanger +11 more
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Quantitative evaluation of embedding resins for volume electron microscopy [PDF]
Frontiers in NeuroscienceOptimal epoxy resin embedding is crucial for obtaining consistent serial sections from large tissue samples, especially for block faces spanning >1 mm2.
Lennart Tegethoff, Kevin L. Briggman
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Volume electron microscopy reveals bacterial endosymbiosis within host mitochondria [PDF]
Communications BiologyBacterial endosymbionts can inhabit certain eukaryotic compartments, yet their direct associations with mitochondria have remained poorly understood. Using a multiscale volumetric electron microscopy (vEM) framework spanning whole-cell reconstructions to
Jiří Týč +13 more
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vEMRec: High‐Resolution Volume Electron Microscopy Reconstruction Based on Structure‐Preserving and High‐Fidelity 3D Alignment [PDF]
Advanced ScienceThree‐dimensional (3D) alignment is a key step in volume electron microscopy (vEM), aimed at addressing misalignment during data acquisition, thereby recovering the correct biological structures.
Zhenbang Zhang +7 more
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Single-Neuron Labeling in Fixed Tissue and Targeted Volume Electron Microscopy [PDF]
Frontiers in Neuroanatomy, 2022 The structural complexity of nervous tissue makes it very difficult to unravel the connectivity between neural elements at different scales. Numerous methods are available to trace long-range projections at the light microscopic level, and to identify ...
Marta Turegano-Lopez +12 more
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VesiclePy: A machine learning vesicle analysis toolbox for volume electron microscopy. [PDF]
PLoS Computational BiologyVesicles are critical components of neurons that package neurotransmitters and neuropeptides for their release, in order to communicate with other neurons and cells.
Jason Ken Adhinarta +10 more
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Three-dimensional reconstruction of rat sperm using volume electron microscopy [PDF]
Acta Biochimica et Biophysica SinicaThree-dimensional (3D) reconstruction serves as a crucial instrument for the analysis of biological structures. In particular, a comprehensive and accurate 3D ultrastructural examination of rat sperm is vital for understanding and diagnosing male ...
Liu Jiazheng +7 more
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Volume electron microscopy reveals age-related circuit remodeling in the auditory brainstem [PDF]
Frontiers in Cellular Neuroscience, 2022 The medial nucleus of the trapezoid body (MNTB) is an integral component of the auditory brainstem circuitry involved in sound localization. The giant presynaptic nerve terminal with multiple active zones, the calyx of Held (CH), is a hallmark of this ...
Daniela Chequer Charan +7 more
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“Orphan” Connexin43 in Plakophilin-2 Deficient Hearts Revealed by Volume Electron Microscopy [PDF]
Frontiers in Cell and Developmental Biology, 2022 Previous studies revealed an abundance of functional Connexin43 (Cx43) hemichannels consequent to loss of plakophilin-2 (PKP2) expression in adult murine hearts.
Chantal J. M. van Opbergen +5 more
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