Results 21 to 30 of about 10,863,365 (261)

Accelerating data sharing and reuse in volume electron microscopy

Nature Cell Biology
Volume electron microscopy (vEM) generates large 3D volumes of cells or tissues at nanoscale resolutions, enabling analyses of organelles in their cellular environment.
Kirk James Czymmek, Ilya Belevich, Johanna Bischof, Aastha Mathur, Lucy Collinson, Eija Jokitalo   +5 more
openaire   +3 more sources

Tracing nerve fibers with volume electron microscopy to quantitatively analyze brain connectivity [PDF]

Communications Biology
The highly complex structure of the brain requires an approach that can unravel its connectivity. Using volume electron microscopy and a dedicated software we can trace and measure all nerve fibers present within different samples of brain tissue.
Marta Turegano-Lopez, Felix de las Pozas, Andrea Santuy, Jose-Rodrigo Rodriguez, Javier DeFelipe, Angel Merchan-Perez   +5 more
doaj   +2 more sources

Diffusion-based deep learning method for augmenting ultrastructural imaging and volume electron microscopy [PDF]

Nature Communications
Electron microscopy (EM) revolutionized the way to visualize cellular ultrastructure. Volume EM (vEM) has further broadened its three-dimensional nanoscale imaging capacity.
Chixiang Lu, Kai Chen, Heng Qiu, Xiaojun Chen, Gu Chen, Xiaojuan Qi, Haibo Jiang   +6 more
doaj   +2 more sources

Biomedical and biological applications of scanning electron microscopy [PDF]

, 2012
Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166This article summarizes the basic principles of scanning electron microscopy and the capabilities of the technique with different examples of applications in biomedical and biological ...
Cortadellas i Raméntol, Núria, García, Almudena, Fernández, Eva   +2 more
core   +6 more sources

Organoids: Unveiling Insights with Volume Electron Microscopy [PDF]

BIO Web of Conferences
Beng Lu Thon, Racine Victor, Beghin Anne, Guerin-Bonne Isabelle   +3 more
doaj   +2 more sources

Transmission electron microscopy in cell biology: sample preparation techniques and image information [PDF]

, 2012
Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166Transmission electron microscopy is a proven technique in the field of cell biology and a very useful tool in biomedical research. Innovation and improvements in equipment together with
Cortadellas i Raméntol, Núria, García, Almudena, Fernández, Eva   +2 more
core   +5 more sources

Genetically encoded barcodes for correlative volume electron microscopy. [PDF]

Nat Biotechnol, 2023
Abstract While genetically encoded reporters are common for fluorescence microscopy, equivalent multiplexable gene reporters for electron microscopy (EM) are still scarce. Here, by installing a variable number of fixation-stable metal-interacting moieties in the lumen of encapsulin nanocompartments of different ...
Sigmund F, Berezin O, Beliakova S, Magerl B, Drawitsch M, Piovesan A, Gonçalves F, Bodea SV, Winkler S, Bousraou Z, Grosshauser M, Samara E, Pujol-Martí J, Schädler S, So C, Irsen S, Walch A, Kofler F, Piraud M, Kornfeld J, Briggman K, Westmeyer GG.   +21 more
europepmc   +8 more sources

Microtubule Tracking in Electron Microscopy Volumes [PDF]

, 2020
Accepted at MICCAI ...
Nils Eckstein, Julia M. Buhmann, Matthew Cook 0001, Jan Funke   +3 more
openaire   +2 more sources

Integrated Array Tomography for 3D Correlative Light and Electron Microscopy

Frontiers in Molecular Biosciences, 2022
Volume electron microscopy (EM) of biological systems has grown exponentially in recent years due to innovative large-scale imaging approaches. As a standalone imaging method, however, large-scale EM typically has two major limitations: slow rates of ...
Ryan Lane, Anouk H. G. Wolters, Ben N. G. Giepmans, Jacob P. Hoogenboom   +3 more
doaj   +1 more source

High-precision targeting workflow for volume electron microscopy [PDF]

Journal of Cell Biology, 2021
Cells are 3D objects. Therefore, volume EM (vEM) is often crucial for correct interpretation of ultrastructural data. Today, scanning EM (SEM) methods such as focused ion beam (FIB)–SEM are frequently used for vEM analyses. While they allow automated data acquisition, precise targeting of volumes of interest within a large sample remains challenging ...
Paolo Ronchi, Giulia Mizzon, Pedro Machado, Edoardo D’Imprima, Benedikt T. Best, Lucia Cassella, Sebastian Schnorrenberg, Marta G. Montero, Martin Jechlinger, Anne Ephrussi, Maria Leptin, Julia Mahamid, Yannick Schwab   +12 more
openaire   +2 more sources