Cells, 2023 (1) Background: Owing to its ready availability and ease of acquisition, developing chick corneal tissue has long been used for research purposes. Here, we seek to ascertain the three-dimensional microanatomy and spatiotemporal interrelationships of the ...
Kiranjit K. Bains +4 more
doaj +1 more source
Optimization of negative stage bias potential for faster imaging in large-scale electron microscopy
Journal of Structural Biology: X, 2021 Large-scale electron microscopy (EM) allows analysis of both tissues and macromolecules in a semi-automated manner, but acquisition rate forms a bottleneck.
Ryan Lane +8 more
doaj +1 more source
Volume electron microscopy for neuronal circuit reconstruction [PDF]
Current Opinion in Neurobiology, 2012 The last decade has seen a rapid increase in the number of tools to acquire volume electron microscopy (EM) data. Several new scanning EM (SEM) imaging methods have emerged, and classical transmission EM (TEM) methods are being scaled up and automated. Here we summarize the new methods for acquiring large EM volumes, and discuss the tradeoffs in terms ...
Briggman, Kevin L., Bock, Davi D.
openaire +5 more sources
3D solutions in transmission electron (Cryo) -microscopy in biology [PDF]
, 2012 Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166One of the main problems in transmission electron microscopy in the biological field is the tri-dimensionality.
Muela, Yolanda +4 more
core +3 more sources
Advanced applications of scanning electron microscopy in geology [PDF]
, 2012 Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166Nowadays Scanning Electron Microscopy (SEM) is a basic and fundamental tool in the study of geologic samples.
Prats Miralles, Eva +3 more
core +5 more sources
Three-Dimensional Structure of Dendritic Spines Revealed by Volume Electron Microscopy Techniques
Frontiers in Neuroanatomy, 2021 Electron microscopy (EM)-based synaptology is a fundamental discipline for achieving a complex wiring diagram of the brain. A quantitative understanding of synaptic ultrastructure also serves as a basis to estimate the relative magnitude of synaptic ...
Laxmi Kumar Parajuli +2 more
doaj +1 more source
Deep generative modeling for volume reconstruction in cryo-electron microscopy [PDF]
Journal of Structural Biology, 2022 Recent breakthroughs in high-resolution imaging of biomolecules in solution with cryo-electron microscopy (cryo-EM) have unlocked new doors for the reconstruction of molecular volumes, thereby promising further advances in biology, chemistry, and pharmacological research.
Donnat, Claire +4 more
openaire +4 more sources
Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes [PDF]
, 2020 Three-dimensional correlative light and electron microscopy (3D CLEM) is attaining popularity as a potential technique to explore the functional aspects of a cell together with high-resolution ultrastructural details across the cell volume.
Peurla , Markus +28 more
core +1 more source
Transfer of tilted sample information in transmission electron microscopy [PDF]
, 2010 When a transmission electron microscope is used in imaging mode, information carried by the sample function is transformed by the optics of the instrument during the imaging process.
Mariani, Valerio
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How innovations in methodology offer new prospects for volume electron microscopy. [PDF]
J Microsc, 2022 Detailed knowledge of biological structure has been key in understanding biology at several levels of organisation, from organs to cells and proteins. Volume electron microscopy (volume EM) provides high resolution 3D structural information about tissues
Kievits AJ +3 more
europepmc +2 more sources