Results 11 to 20 of about 10,863,365 (261)

Volume electron microscopy. [PDF]

Nat Rev Methods Primers, 2022
Life exists in three dimensions, but until the turn of the century most electron microscopy methods provided only 2D image data. Recently, electron microscopy techniques capable of delving deep into the structure of cells and tissues have emerged, collectively called volume electron microscopy (vEM).
Peddie CJ, Genoud C, Kreshuk A, Meechan K, Micheva KD, Narayan K, Pape C, Parton RG, Schieber NL, Schwab Y, Titze B, Verkade P, Aubrey A, Collinson LM.   +13 more
europepmc   +9 more sources

SyConn2: dense synaptic connectivity inference for volume electron microscopy. [PDF]

Nat Methods, 2022
AbstractThe ability to acquire ever larger datasets of brain tissue using volume electron microscopy leads to an increasing demand for the automated extraction of connectomic information. We introduce SyConn2, an open-source connectome analysis toolkit, which works with both on-site high-performance compute environments and rentable cloud computing ...
Schubert PJ, Dorkenwald S, Januszewski M, Klimesch J, Svara F, Mancu A, Ahmad H, Fee MS, Jain V, Kornfeld J.   +9 more
europepmc   +7 more sources

Large Volume Electron Microscopy and Neural Microcircuit Analysis [PDF]

Frontiers in Neural Circuits, 2018
One recent technical innovation in neuroscience is microcircuit analysis using three-dimensional reconstructions of neural elements with a large volume Electron microscopy (EM) data set.
Yoshiyuki Kubota, Yoshiyuki Kubota, Jaerin Sohn, Jaerin Sohn, Yasuo Kawaguchi, Yasuo Kawaguchi   +5 more
doaj   +3 more sources

Editorial: Advances in volume electron microscopy for brain imaging: methods, applications, and affordability [PDF]

Frontiers in Neuroscience
Corrado Calì, Corrado Calì, Xueying Wang   +2 more
doaj   +4 more sources

Exploiting volume electron microscopy to investigate structural plasticity and stability of the postsynaptic compartment of central synapses [PDF]

Frontiers in Cellular Neuroscience, 2023
Volume reconstruction from electron microscopy datasets is a tool increasingly used to study the ultrastructure of the synapse in the broader context of neuronal network and brain organization.
Greta Maiellano, Greta Maiellano, Lucrezia Scandella, Maura Francolini   +3 more
doaj   +2 more sources

FAST-EM array tomography: a workflow for multibeam volume electron microscopy [PDF]

Methods in Microscopy
Elucidating the 3D nanoscale structure of tissues and cells is essential for understanding the complexity of biological processes. Electron microscopy (EM) offers the resolution needed for reliable interpretation, but the limited throughput of electron ...
Kievits Arent J., Duinkerken B. H. Peter, Lane Ryan, de Heus Cecilia, van Beijeren Bergen en Henegouwen Daan, Höppener Tibbe, Wolters Anouk H. G., Liv Nalan, Giepmans Ben N. G., Hoogenboom Jacob P.   +9 more
doaj   +2 more sources

Unambiguous identification of asymmetric and symmetric synapses using volume electron microscopy [PDF]

Frontiers in Neuroanatomy
The brain contains thousands of millions of synapses, exhibiting diverse structural, molecular, and functional characteristics. However, synapses can be classified into two primary morphological types: Gray’s type I and type II, corresponding to ...
Nicolás Cano-Astorga, Nicolás Cano-Astorga, Nicolás Cano-Astorga, Nicolás Cano-Astorga, Sergio Plaza-Alonso, Sergio Plaza-Alonso, Sergio Plaza-Alonso, Marta Turegano-Lopez, Marta Turegano-Lopez, Marta Turegano-Lopez, José Rodrigo-Rodríguez, José Rodrigo-Rodríguez, José Rodrigo-Rodríguez, Angel Merchan-Perez, Angel Merchan-Perez, Angel Merchan-Perez, Javier DeFelipe, Javier DeFelipe, Javier DeFelipe   +18 more
doaj   +2 more sources

Cellular Hallmarks From Volume Electron Microscopy Reveal Developmental Progression of Plasmodium Ookinetes [PDF]

Advanced Science
Unicellular organisms or cells of metazoans often change their morphology during development or life cycle progression to adapt to environmental changes. Malaria parasites undergo a striking range of morphological transformations as they navigate through
Nedal Darif, Marco Rheinnecker, Kolja Hildenbrand, Thanat Chookajorn, Lilian P. Dorner, Jean‐Karim Hériché, Sara Henriksson, Charlotta Funaya, Franziska Hentzschel, Linda Sandblad, Oliver Billker, Yannick Schwab, Friedrich Frischknecht   +12 more
doaj   +2 more sources

vEMINR: Ultra‐Fast Isotropic Reconstruction for Volume Electron Microscopy With Implicit Neural Representation [PDF]

Advanced Science
Volume electron microscopy (vEM) is a powerful technique that enables 3D visualization of biological structures at the nanometer scale. However, vEM imaging relies on sequential scanning of 2D images, and due to section thickness limitations, the axial ...
Jibin Yang, Jie Huo, Muyu Liu, Chenjie Feng, Yan Zhang, Gang Pan, Wenjia Meng, Renmin Han   +7 more
doaj   +2 more sources

Volume electron microscopy reveals unique laminar synaptic characteristics in the human entorhinal cortex [PDF]

eLife
The entorhinal cortex (EC) plays a pivotal role in memory function and spatial navigation, connecting the hippocampus with the neocortex. The EC integrates a wide range of cortical and subcortical inputs, but its synaptic organization in the human brain ...
Sergio Plaza-Alonso, Nicolas Cano-Astorga, Javier DeFelipe, Lidia Alonso-Nanclares   +3 more
doaj   +2 more sources