Results 1 to 10 of about 10,640,977 (121)

FIB-SEM as a Volume Electron Microscopy Approach to Study Cellular Architectures in SARS-CoV-2 and Other Viral Infections: A Practical Primer for a Virologist

Viruses, 2021
The visualization of cellular ultrastructure over a wide range of volumes is becoming possible by increasingly powerful techniques grouped under the rubric “volume electron microscopy” or volume EM (vEM).
Valentina Baena, Ryan Conrad, Patrick Friday, Ella Fitzgerald, Taeeun Kim, John Bernbaum, Heather Berensmann, Adam Harned, Kunio Nagashima, Kedar Narayan   +9 more
doaj   +4 more sources

vEMINR: Ultra‐Fast Isotropic Reconstruction for Volume Electron Microscopy With Implicit Neural Representation [PDF]

Advanced Science
Volume electron microscopy (vEM) is a powerful technique that enables 3D visualization of biological structures at the nanometer scale. However, vEM imaging relies on sequential scanning of 2D images, and due to section thickness limitations, the axial ...
Jibin Yang, Jie Huo, Muyu Liu, Chenjie Feng, Yan Zhang, Gang Pan, Wenjia Meng, Renmin Han   +7 more
doaj   +3 more sources

vEMRec: High‐Resolution Volume Electron Microscopy Reconstruction Based on Structure‐Preserving and High‐Fidelity 3D Alignment [PDF]

Advanced Science
Three‐dimensional (3D) alignment is a key step in volume electron microscopy (vEM), aimed at addressing misalignment during data acquisition, thereby recovering the correct biological structures.
Zhenbang Zhang, Hongjia Li, Zhongjun Yang, Zhiqiang Xu, Duanchen Sun, Xin Gao, Fa Zhang, Renmin Han   +7 more
doaj   +3 more sources

Cellular Hallmarks From Volume Electron Microscopy Reveal Developmental Progression of Plasmodium Ookinetes [PDF]

Advanced Science
Unicellular organisms or cells of metazoans often change their morphology during development or life cycle progression to adapt to environmental changes. Malaria parasites undergo a striking range of morphological transformations as they navigate through
Nedal Darif, Marco Rheinnecker, Kolja Hildenbrand, Thanat Chookajorn, Lilian P. Dorner, Jean‐Karim Hériché, Sara Henriksson, Charlotta Funaya, Franziska Hentzschel, Linda Sandblad, Oliver Billker, Yannick Schwab, Friedrich Frischknecht   +12 more
doaj   +3 more sources

Diffusion-based deep learning method for augmenting ultrastructural imaging and volume electron microscopy

Nature Communications
Electron microscopy (EM) revolutionized the way to visualize cellular ultrastructure. Volume EM (vEM) has further broadened its three-dimensional nanoscale imaging capacity.
Chixiang Lu, Kai Chen, Heng Qiu, Xiaojun Chen, Gu Chen, Xiaojuan Qi, Haibo Jiang   +6 more
doaj   +3 more sources

Editorial: Advances in volume electron microscopy for brain imaging: methods, applications, and affordability [PDF]

Frontiers in Neuroscience
Corrado Calì, Corrado Calì, Xueying Wang   +2 more
doaj   +4 more sources

Protocol for fixation and staining of postmortem human brain tissue optimized for focused ion beam-scanning electron microscopy [PDF]

STAR Protocols
Summary: Here, we present a protocol for generating postmortem human brain tissue samples suitable for volume electron microscopy (vEM) imaging via focused ion beam-scanning electron microscopy (FIB-SEM).
Matthew J. Maier, David A. Lewis, Zachary Freyberg, Jill R. Glausier   +3 more
doaj   +2 more sources

VesiclePy: A machine learning vesicle analysis toolbox for volume electron microscopy. [PDF]

PLoS Computational Biology
Vesicles are critical components of neurons that package neurotransmitters and neuropeptides for their release, in order to communicate with other neurons and cells.
Jason Ken Adhinarta, Yutian Fan, Adam Gohain, Michael Lin, Paige Nurkin, Richard Ren, Micaela Roth, Shulin Zhang, Ayal Yakobe, Rafael Yuste, Donglai Wei   +10 more
doaj   +2 more sources

Exploiting volume electron microscopy to investigate structural plasticity and stability of the postsynaptic compartment of central synapses

Frontiers in Cellular Neuroscience, 2023
Volume reconstruction from electron microscopy datasets is a tool increasingly used to study the ultrastructure of the synapse in the broader context of neuronal network and brain organization.
Greta Maiellano, Greta Maiellano, Lucrezia Scandella, Maura Francolini   +3 more
doaj   +3 more sources

Efficient cell-wide mapping of mitochondria in electron microscopic volumes using webKnossos [PDF]

Cell Reports: Methods
Summary: Recent technical advances in volume electron microscopy (vEM) and artificial-intelligence-assisted image processing have facilitated high-throughput quantifications of cellular structures, such as mitochondria, that are ubiquitous and ...
Yi Jiang, Haoyu Wang, Kevin M. Boergens, Norman Rzepka, Fangfang Wang, Yunfeng Hua   +5 more
doaj   +2 more sources