Results 21 to 30 of about 10,640,977 (121)

Getting Started in vEM

open access: yes, 2022
A starter toolkit for learning volume electron microscopy techniques, created by the training working group of the vEM Community ...
Louise Hughes
core   +1 more source

Transmission electron microscopy in cell biology: sample preparation techniques and image information [PDF]

open access: yes, 2012
Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166Transmission electron microscopy is a proven technique in the field of cell biology and a very useful tool in biomedical research. Innovation and improvements in equipment together with
Cortadellas i Raméntol, Núria   +2 more
core   +5 more sources

One for All, All for One: A Close Look at In-Resin Fluorescence Protocols for CLEM

open access: yesFrontiers in Cell and Developmental Biology, 2022
Sample preparation is the novel bottleneck for high throughput correlative light and electron microscopy (CLEM). Protocols suitable for both imaging methods must therefore balance the requirements of each technique.
Xavier Heiligenstein, Miriam S. Lucas
doaj   +1 more source

Advances in High-Resolution Cryo Volume Electron Microscopy (cvEM) Imaging for Unicellular and Multicellular Organisms

open access: yesJournal of Microscopy, EarlyView.
Abstract Cryo‐Focused Ion Beam Scanning Electron Microscopy (cryoFIB‐SEM) using samples fixed by high‐pressure freezing uniquely enables high‐resolution cryo‐volume Electron Microscope (cvEM) images of cell ultrastructure to be obtained from whole cells and complex tissues in their near native state. As the freezing process also preserves fluorescence,
Kobylynska M   +10 more
europepmc   +2 more sources

3D solutions in transmission electron (Cryo) -microscopy in biology [PDF]

open access: yes, 2012
Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166One of the main problems in transmission electron microscopy in the biological field is the tri-dimensionality.
Muela, Yolanda   +4 more
core   +3 more sources

Volume EM: A quiet revolution takes shape [PDF]

open access: yes, 2023
Volume electron microscopy (vEM) is a group of techniques that reveal the 3D ultrastructure of cells and tissues through continuous depths of at least 1 micrometer.
Carzaniga, Raffaella   +15 more
core   +4 more sources

Resin comparison for serial block face scanning volume electron microscopy

open access: yes, 2023
Serial Block Face Scanning Electron Microscopy (SBF-SEM) is one of several volume electron microscopy (vEM) techniques whose purpose is to reveal the nanostructure of cells and tissues in three dimensions. As one of the earliest, and possibly most widely
Borghgraef, Peter   +9 more
core   +1 more source

A Pipeline for Volume Electron Microscopy of the Caenorhabditis elegans Nervous System

open access: yesFrontiers in Neural Circuits, 2018
The “connectome,” a comprehensive wiring diagram of synaptic connectivity, is achieved through volume electron microscopy (vEM) analysis of an entire nervous system and all associated non-neuronal tissues. White et al.
Ben Mulcahy   +12 more
doaj   +1 more source

Transfer of tilted sample information in transmission electron microscopy [PDF]

open access: yes, 2010
When a transmission electron microscope is used in imaging mode, information carried by the sample function is transformed by the optics of the instrument during the imaging process.
Mariani, Valerio
core   +1 more source

Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes [PDF]

open access: yes, 2020
Three-dimensional correlative light and electron microscopy (3D CLEM) is attaining popularity as a potential technique to explore the functional aspects of a cell together with high-resolution ultrastructural details across the cell volume.
Peurla , Markus   +28 more
core   +1 more source

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