Results 101 to 110 of about 6,718,063 (241)

Life cycle cost-effectiveness estimation and optimization method for satellite [PDF]

Zhihui kongzhi yu fangzhen
In order to design satellite with low cost and high effectiveness, an optimization method of life cycle cost-effectiveness for satellites is proposed in this paper.
ZHAO Jianyu, WANG Jingyan, CHENG Zhuo, JIAO Jian, CHENG Yao
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Economic Evaluation of the Metabolic and Bariatric Surgery Initiative for Patients With Obesity and Comorbid Diabetes in Queensland

Obesity Science & Practice
Background Metabolic and bariatric surgery is the most effective long‐term treatment for patients with obesity; however, access to publicly funded surgery in Australia has been limited despite strong evidence of clinical benefit.
Paul Scuffham, Katie Wykes, Jody Paxton
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Cost-effectiveness acceptability curve.

, 2020
Cost-effectiveness acceptability curve.
Mercy Mvundura (701688), Fakhr Abu-Awwad (8407371), Assad Ramlawi (8407377), Frédéric Debellut (5439416), Clint Pecenka (835779), Mustafa Barham (8407368), Samer Jaber (8407359), Andrew Clark (279535), Jehad Sabbah (8407365), Yaser Bouzya (8407362), Diaa Hjaija (8407374)   +10 more
core   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis

FEBS Open Bio, EarlyView.
MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss, Ao Li, Eduardo H. Gilglioni, Mayank Bansal, Sumeet Pal Singh, Latifa Bakiri, Alessandra K. Cardozo, Esteban N. Gurzov   +7 more
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Towards a cost-effectiveness analysis of the measurement of biodiversity indicators [PDF]

A comprehensive quantification of biodiversity in farming systems would require a very significant amount of work (and funds) even for a small area. Therefore, biodiversity indicators are needed to solve the problem of the measurement feasibility.
Viaggi, Davide, Targetti, Stefano, Cuming, David   +2 more
core