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Integration of Single Pass Tangential Flow Filtration and High Performance Countercurrent Membrane Purification for Intensification of Monoclonal Antibody Processing. [PDF]
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Journal of Proteomics, 1981
Countercurrent chromatography is a newly developed versatile partition chromatography which totally eliminates the use of solid supports. The method utilizes the intriguing hydrodynamic behavior of two immiscible solvents in a rotating coiled tube. Various types of seal-free flow-through centrifuge schemes are introduced to facilitate a continuous ...
Yoichirô Itô
exaly +4 more sources
Countercurrent chromatography is a newly developed versatile partition chromatography which totally eliminates the use of solid supports. The method utilizes the intriguing hydrodynamic behavior of two immiscible solvents in a rotating coiled tube. Various types of seal-free flow-through centrifuge schemes are introduced to facilitate a continuous ...
Yoichirô Itô
exaly +4 more sources
Gradient Elution in Countercurrent Chromatography
Planta Medica, 2015Countercurrent chromatography is a form of liquid-liquid partition chromatography in which the stationary liquid phase is retained in the apparatus without the use of a solid support. Gradient elution in countercurrent chromatography can be used in many different ways, such as linear gradients, stepwise elution gradients, pH gradients, etc.
Gilda Leitao
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pH-zone-refining countercurrent chromatography
Journal of Chromatography A, 1996A recently developed preparative technique, pH-zone-refining countercurrent chromatography (CCC), separates organic acids and bases according to their pKa and hydrophobicity. The hydrodynamic mechanism of pH-zone-refining CCC is illustrated in two elution modes along with a simple mathematical analysis.
Yoichirô Itô
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Droplet Countercurrent Chromatography
Science, 1970A new form of countercurrent chromatography, named droplet countercurrent chromatography, has been developed. This all-liquid separation technique is based on the partitioning of solute between a steady stream of droplets of moving phase and a column of surrounding stationary liquid phase.
T, Tanimura +3 more
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Chemical Engineering Science, 1988
Abstract A continuous countercurrent moving bed chromatographic reactor, and a simulated countercurrent moving bed apparatus have been interfaced with a laboratory microcomputer for data acquisition and computer control. Simulation of a countercurrent moving bed is accomplished by switching a feedstream sequentially through a series of columns ...
Barry B. Fish +2 more
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Abstract A continuous countercurrent moving bed chromatographic reactor, and a simulated countercurrent moving bed apparatus have been interfaced with a laboratory microcomputer for data acquisition and computer control. Simulation of a countercurrent moving bed is accomplished by switching a feedstream sequentially through a series of columns ...
Barry B. Fish +2 more
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Countercurrent chromatography: People and applications
Journal of Chromatography A, 2009The scientific literature was scanned for the published research articles dealing with countercurrent chromatography (CCC) over the time period 1980-May 2008. The search returned 1638 articles that were analyzed focussing on people and applications. Concerning the people, it was found that the geographical location of the CCC authors was relatively ...
A, Berthod +2 more
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Countercurrent chromatography meets MS
Nature, 1989Interfacing countercurrent chromatography with thermospray mass spectrometry provides a new analytical tool for detecting nonvolatile, hydrophilic or thermally unstable bioactive natural products.
Y W, Lee, R D, Voyksner
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Recycling Foam Countercurrent Chromatography
Analytical Chemistry, 2000A new sample injection method for foam countercurrent chromatography (CCC), named the "recycle injection system", has been developed. In this system, the effluent from the liquid outlet is directly returned into the column through the sample feed line so that the sample solution is continuously recycled for repetitive foam fractionation. The utility of
, Oka +4 more
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High-Speed Countercurrent Chromatography
Critical Reviews in Analytical Chemistry, 1986I. INTRODUCTION During the past 20 years countercurrent chromatography (CCC) has been steadily improved in both partition efficiency and separation times.1-15 The original helix CCC and the successively developed nonhelix preparative-scale CCC schemes, such as droplet CCC16,17 and locular CCC,17 required relatively long separating times; sizeable ...
Yoichiro Ito, Walter D. Conway
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