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Progress of chiral countercurrent chromatography
Chinese Journal of Chromatography, 2016This article summarizes five characteristics of chiral countercurrent chromatography (CCC). The progress of chiral countercurrent chromatography is introduced. In the high speed countercurrent chromatography, the chiral selectors such as amino acid derivatives, cyclodextrin derivatives, chiral organic acid, polysaccharide and bovine serum albumin (BSA)
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Advances in Countercurrent Chromatography For Protein Separations
Bioanalysis, 2012Countercurrent chromatography (CCC) is a preparative purification technique working with biphasic liquid systems. One phase of the liquid system is selected to be the mobile phase, the other phase is the stationary phase, held still by centrifugal fields. Aqueous two-phase systems (ATPS) have demonstrated their use in biological purifications.
Mekaoui, Nazim +2 more
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Dual Countercurrent Chromatography
Journal of Liquid Chromatography, 1988Abstract A modern method of performing conventional countercurrent distribution called Dual Countercurrent Chromatography is described. The system consists of a multilayer coiled column integrated with two inlet and two outlet lines for solvent phases and a sample feed line. Subjecting the system to a particular combination of centrifugal and planetary
Y.-W. Lee, C. E. Cook, Y. Ito
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multifraction separation in countercurrent chromatography (MCSGP)
Biotechnology and Bioengineering, 2013ABSTRACTThe multicolumn countercurrent solvent gradient purification (MCSGP) process is a continuous countercurrent multicolumn chromatography process capable of performing three fraction separations while applying a linear gradient of some modifier.
M. Krättli +2 more
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Resolution in Countercurrent Chromatography
Journal of Liquid Chromatography, 1985Abstract Using as an example the separation of DNP-glutamic acid and DNP-alanine in a nonplanetary countercurrent chromatograph, it was demonstrated that resolution, Rs, of a solute pair can be satisfactorily predicted from knowledge of the column efficiency, N, the fraction of column volume occupied by stationary phase, SF, and the partition ...
Walter D. Conway, Yoichiro Ito
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High-speed countercurrent chromatography
Nature, 1987Support-free high-speed countercurrent liquid chromatography provides a rich domain of applications, some beyond reach of conventional liquid chromatography.
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Chiral Separations by Countercurrent Chromatography
2019Chiral separations by countercurrent chromatography are mainly divided into two types: homogeneous chiral selector addition and interfacial chiral ligand exchange. In this chapter, we describe two methods for the enantioseparation of phenylsuccinic acid and α-hydroxy acids by high-speed countercurrent chromatography using hydroxypropyl-β-cyclodextrin ...
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Comparison of Countercurrent Chromatography with Flash Chromatography
Journal of Liquid Chromatography & Related Technologies, 1998Abstract Studies of the separation of benzyl, phenylethyl and phenylpropyl alcohols by flash chromatography (FC) on 1.0 × 30 or 1.9 × 15 cm silica gel columns are compared with separation by countercurrent chromatography (CCC) on a 1.68 mm × 24 m, 56 mL, PTFE multilayer coil.
W. D. Conway +3 more
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Trends in countercurrent chromatography
TrAC Trends in Analytical Chemistry, 1986Abstract Since the early 1970s countercurrent chromatography has achieved a remarkable advance and is currently yielding rapid and efficient chromatographic separations that are comparable with those obtained with preparative high-performance liquid chromatography.
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Development of High-Speed Countercurrent Chromatography
2021The preparative capability of the multilayer coil has been demonstrated in the separation of 1 g of the DNP amino acid mixture under the optimal operational conditions determined by the preliminary studies on short coils as described above. These centrifuge schemes are equipped with a novel flow-through mechanism which entirely eliminates the need for ...
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