Results 111 to 120 of about 299,628 (304)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Investigation of the power coupling of novel wavelength-selective couplers incorporating axially symmetric long-period fibre gratings

open access: yesSouth African Journal of Science, 2011
Evanescent-field coupling was studied experimentally in novel optical-fibre-based wavelength-selective couplers, using axially symmetric long-period fibre grating (LPFG) structures.
Ronnie Kritzinger   +2 more
doaj  

Study on the Influence of Atmospheric Light Intensity Scintillation Effect on Optical Fiber Coupling Efficiency

open access: yesPhotonics
Light intensity flicker is the most basic and important effect of turbulence. Fiber coupling efficiency is the primary parameter that ensures the system’s communication quality.
Xiaoying Ding, Xin Zhao
doaj   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

open access: yesFEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada   +5 more
wiley   +1 more source

Off-axis injection for storage rings with full coupling

open access: yesPhysical Review Accelerators and Beams, 2018
The next generation of storage ring light sources will likely operate with high linear coupling, which could potentially prevent the use of off-axis injection as large horizontal oscillation of the injected beam is coupled to the vertical plane.
Tong Zhang, Xiaobiao Huang
doaj   +1 more source

Unidirectional, efficiency-controlled coupling from microcavity using reflection feedback

open access: yes, 2007
We propose a microcavity-to-waveguide side coupling system with a reflector at one end of the waveguide for the feedback. Using this system, one can get a unidirectional output from a microcavity-based light emitter, where the output coupling efficiency ...
Lee, Yong-Hee, Hwang, IK
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

open access: yesFEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee   +6 more
wiley   +1 more source

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