Heat Shock Protein Family A Member 1 Promotes Intracellular Amplification of Hepatitis B Virus Covalently Closed Circular DNA. [PDF]
J Virol, 2023 Tang L +5 more
europepmc +1 more source
Advanced Science, EarlyView.Engineered Escherichia coli Nissle 1917 with a tannic acid‐mediated BPA coating enables tumor‐targeted boron delivery for BNCT. The low‐level incidental radiation generated during BNCT activate a RecA‐driven circuit to induce CXCL10, while radiation‐enhanced bacterial colonization triggers in situ vaccination and systemic antitumor immunity.
Yang Liu +11 more
wiley +1 more source
A New Model to Assess Hepatitis B Virus Covalently Closed Circular DNA: A Window Into a Previously Hidden Space? [PDF]
Cell Mol Gastroenterol Hepatol, 2022 Xu M, Lin W, Chung RT.
europepmc +1 more source
Advanced Science, EarlyView.This work develops a metabolic sharing strategy that employs cytidine monophosphate (CMP) to competitively reprogram floxuridine (FUDR) pyrimidine metabolism in colorectal tumor cells. Elevated intracellular FdUMP induces nucleotide imbalance, drives mitochondrial DNA release and cGAS‐STING innate immune cascade activation, and potentiates robust ...
Chao Wang +10 more
wiley +1 more source
Conserved Lysine Residues of Hepatitis B Virus Core Protein Are Not Required for Covalently Closed Circular DNA Formation. [PDF]
J Virol, 2022 Hong X, Hu J.
europepmc +1 more source
A simple large-scale method for separating closed circular form DNA by gel electrophoresis
The covalently closed form of circular duplex SV40 DNA was separated from the open and linear form of SV40 DNA by agarose gel electrophoresis in a large-scale gel system.
Oda, Takuzo +3 more
core
Reversible, Chemically Gated FRET via Ligand‐Activated Acceptors
Advanced Science, EarlyView.Fluorogen binding turns the compact FAST tag into a tunable FRET acceptor, with ligand chemistry programming spectral overlap and excited‐state lifetimes. Fluorescence‐lifetime imaging reads out energy transfer through the shortened donor lifetime, while simply adding or washing out the dye switches FRET on and off, reversibly mapping the supramodular ...
Nivedita Singh +7 more
wiley +1 more source
A simple method for the preparation of the covalently closed circular form of plasmid DNA.
BioTechniques, 1990 We describe a simple, rapid, inexpensive method for isolation of covalently closed circular plasmid DNA. The method involves the electrophoresis of crude DNA preparations in an agarose gel, electrotransfer onto a dialysis membrane and elution of the highly purified circular covalently closed plasmid DNA.
J J, Le Brun +2 more
openaire +1 more source
Advanced Science, EarlyView.The SPG‐MpECis electrochemical POCT platform developed in this study enables direct detection of biomarkers in complex biological samples such as serum and fingerstick blood, demonstrating high concordance with results from clinical reference methods and holding broad potential for at‐home testing applications.
Menghan Li +10 more
wiley +1 more source
Screening of an epigenetic compound library identifies BRD4 as a potential antiviral target for hepatitis B virus covalently closed circular DNA transcription. [PDF]
Antiviral Res, 2023 Yu X +11 more
europepmc +1 more source