Results 61 to 70 of about 2,023,461 (294)

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Grazing and cover crop effects on soil health during transition to organic production

Agrosystems, Geosciences & Environment
The authors studied the efficacy of six objective‐based, reduced tillage cover cropping systems with or without livestock grazing to induce changes in soil condition and soil health on a fine sandy loam during the organic transition phase in the northern
Abmael S. Cardoso, Mark A. Liebig, John R. Hendrickson, Scott L. Kronberg, Andrea K. Clemensen, David W. Archer, Christine Nieman, Marta M. Kohmann, José G. Franco   +8 more
doaj   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Effects of the second crop on maize yield and yield components in organic agriculture [PDF]

, 2008
The second crop use in organic agriculture is a known method of maintaining the soil tilth, soil protection against environmental deterioration, soil nutrients conservation and even the weed control.
Jug, I., Samota, D., Vrkljan, B., Stipesevic, B., Kolar, D., Birkás, Márta, Jug, D.   +6 more
core   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Cover Crop Adoption and Disadoption: The Role of Agricultural Policies, Climate, and Regional Dynamics

Despite cover crops been widely promoted for their on and off-farm benefits, their adoption is both low nationally and diminishing in some areas. Existing research has examined a suite of factors affecting the adoption of cover crops in the United States,
Elizabeth Espinosa-Uquillas, Meredith Niles, Roderick M. Rejesus   +2 more
core   +5 more sources

Cover Crop Biomass Production Is More Important than Diversity for Weed Suppression [PDF]

, 2019
Biotic resistance theory suggests that diverse cover crop mixes may be more effective at weed suppression than a cover crop monoculture. However, evidence for this has so far been inconsistent.
Bennett, James, James Bennett, Wright, J., Dehnen-Schmutz, K., Bennett, J., MacLaren, Chloe, Pieter Swanepoel, Swanepoel, P., Swanepoel, Pieter, MacLaren, C., Wright, Julia; id_orcid, Chloe MacLaren, Dehnen-Schmutz, Katharina; id_orcid, Katharina Dehnen-Schmutz, Julia Wright   +14 more
core   +1 more source

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

FEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada, Takaaki Tanaka, Manaka Uehara, Keigo Nakano, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Masafumi Yohda   +7 more
wiley   +1 more source

Cover Crop Mixture Effects on Maize, Soybean, and Wheat Yield in Rotation

Agricultural & Environmental Letters, 2019
Despite the popularity of multispecies cover crop mixtures, there is little published evidence of their effects on subsequent crop yields, especially for multiple crops grown in rotation.
Mitchell C. Hunter, Meagan E. Schipanski, Macdonald H. Burgess, James C. LaChance, Brosi A. Bradley, Mary E. Barbercheck, Jason P. Kaye, David A. Mortensen   +7 more
doaj   +1 more source