Shuili Shuiyun Gongcheng XuebaoWith comprehensive seepage monitoring data, traditional methods can effectively provide early warnings for seepage safety. However, after drought conditions, these methods show a delay in accurately reflecting the dam’s seepage status through monitoring ...
YE Wei +4 more
doaj +1 more source
Study on Crack Development of Concrete Lining with Insufficient Lining Thickness Based on CZM Method. [PDF]
Materials (Basel), 2021 Liu J +5 more
europepmc +1 more source
A crack-like notch analogue for a safe-life fretting fatigue design methodology
, 2003 Various analogies have recently been proposed for comparing the stress fields induced in fretting fatigue contact situations, with those of a crack and a sharp or a rounded notch, resulting in a degree of uncertainty over which model is most appropriate ...
Ciavarella, M., M. CIAVARELLA
core +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Study on Crack Development and Micro-Pore Mechanism of Expansive Soil Improved by Coal Gangue under Drying-Wetting Cycles. [PDF]
Materials (Basel), 2021 Zhu H, Zhang Y, Li Z, Xue X.
europepmc +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Characteristics of Moisture Transfer and Surface Crack Development of a Single Lignite Particle Driven by Humidity Difference. [PDF]
ACS Omega, 2021 Gao M +6 more
europepmc +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
IDB Newsletter: Vol. 6, no. 3, July 1968
, 1968 IDB Newsletter is a monthly publication of the Inter-American Development that reports on the activities of the Inter-American Development Bank and appears in English and Spanish ...
Inter-American Development Bank
core +1 more source
Small RNA pathways in mammalian oocytes
FEBS Open Bio, EarlyView.Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley +1 more source