Results 41 to 50 of about 1,668,091 (242)
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
The effect of low-cycle fatigue on evolution of fracture mechanics parameters in residual stress field caused by cold hole expansion [PDF]
Fracture and Structural Integrity, 2019 Localized displacement measurements based on electronic speckle-pattern interferometry are used to obtain crack mouth opening displacement (CMOD), stress intensity factor (SIF) and T-stress values during crack growth around cold-expanded holes.
Yu. G. Matvienko +2 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
A Workflow to Accelerate Microstructure‐Sensitive Fatigue Life Predictions
Advanced Engineering Materials, EarlyView.This study introduces a workflow to accelerate predictions of microstructure‐sensitive fatigue life. Results from frameworks with varying levels of simplification are benchmarked against published reference results. The analysis reveals a trade‐off between accuracy and model complexity, offering researchers a practical guide for selecting the optimal ...
Luca Loiodice +2 more
wiley +1 more source
Experimental evaluation of CTOD in constant amplitude fatigue crack growth from crack tip displacement fields [PDF]
Fracture and Structural Integrity, 2017 In the current work an experimental study of the crack tip opening displacement (CTOD) is performed to evaluate the ability of this parameter to characterise fatigue crack growth.
J.M. Vasco-Olmo +3 more
doaj +1 more source
Current Status and Challenges in Data Collection for Aerospace Coatings Deposited by Plasma Spraying
Advanced Engineering Materials, EarlyView.An innovative approach has been integrated into the GRENAT project to optimize plasma spraying and coating performance. Raw materials are accelerated and melted in the plasma generated by torches, creating coatings. Monitoring sensors collect process data which are combined with ex situ characterization data.
Lila Randriamananjara +8 more
wiley +1 more source
Advanced Engineering Materials, EarlyView.Residual adhesive after electrode loading in adhesive‐assisted resistance spot welding is quantified through a traceable experimental‐to‐digital workflow. Chromatic confocal topography provides calibrated surface‐height data, while OpenCV detects the electrode imprint and integrates adhesive height into comparable volume metrics.
Sung‐Min Wi, Jiangdong Zhao
wiley +1 more source
Adaptive Foam 3D Printing of Ultralight and Multifunctional Materials
Advanced Engineering Materials, EarlyView.Adaptive foam 3D printing, enabled by expandable microspheres, imparts cellular structures to thermoplastic and thermosetting polymers, manufactured through a variety of processes including fused filament fabrication, direct ink writing, digital light processing, and inkjet printing.
Nariman Rajabifar, Amir Ameli
wiley +1 more source
The cohesive frictional crack model applied to the analysis of the dam-foundation joint [PDF]
, 2010 The mechanical behaviour of dam-foundation joints plays a key role in concrete dam engineering since it is the weakest part of the structure and therefore the evolutionary crack process occurring along this joint determines the global load-bearing ...
Barpi, Fabrizio, Valente, Silvio
core +1 more source
Effect of hydration and crack orientation on crack-tip strain, crack opening displacement and crack-tip shielding in elephant dentin [PDF]
, 2018 Objectives. To quantify the extent of crack-tip plasticity, crack opening displacement (COD) and crack bridging for crack growth perpendicular (HAH) and parallel (RAR) to the tubules in elephant dentin under both hydrated and dry conditions to better ...
Rawson, Shelley +8 more
core +2 more sources