Results 201 to 210 of about 895,426 (293)

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Associations Between Ostomy Creation and Health-Related Quality of Life in Colorectal Cancer Patients: A Longitudinal Observational Study. [PDF]

Cancer Med
Lau J, Ng A, Koh WL, Peh CH, Singapore Colorectal Cancer Research Group, Luo N, Tan KK.   +6 more
europepmc   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

Developing and validating the co-creation rainbow framework for intrinsic evaluation of methods: a health CASCADE structured review of models representing co-creation principles. [PDF]

Health Res Policy Syst
Agnello DM, Smith N, Vogelsang M, Steiner A, An Q, de Boer J, Calo F, Delfmann L, Hutcheon D, Longworth GR, Loisel Q, Mazzei M, McCaffrey L, Renzella J, Chastin S.   +14 more
europepmc   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

Molecular determinants of signal transduction in tropomyosin receptor kinases

FEBS Open Bio, EarlyView.
Tropomyosin receptor kinases control critical neuronal functions, but how do the same receptors produce diverse cellular responses? This review explores the structural mechanisms behind Trk signaling diversity, focusing on allosteric modulation and ligand bias.
Giray Enkavi
wiley   +1 more source

Surgical Education for Pressure Injuries: A Survey of What Residents are Learning in Ontario. [PDF]

Plast Surg (Oakv)
Wan H, Laurent R, Rogers A, Wallace D.
europepmc   +1 more source

Edgar Odell Lovett and the creation of Rice University : The meaning of the new institution

, 2000
Edgar Odell Lovett, John B. Boles, Karen Hess Rogers   +2 more
openalex   +1 more source

Long‐term culture of skin biopsies: maintenance of fibroblast production and competency of reprogramming

FEBS Open Bio, EarlyView.
Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar, Michael A. Ripperger, Grant M. Westlake, Kevin C. Ess   +3 more
wiley   +1 more source

Endoscopic ultrasound-guided colorectal anastomosis using a lumen-apposing metal stent for complete anastomotic stricture. [PDF]

Endoscopy
Prado Pessoa R, Chaves CAA, Mafra JGA, Vieira ESC, Mafra LGA, Rocha LCM, Roda R.   +6 more
europepmc   +1 more source