Results 21 to 30 of about 79,886 (234)

Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes. [PDF]

, 2020
Anti-CRISPRs (Acrs) are small proteins that inhibit the RNA-guided DNA targeting activity of CRISPR-Cas enzymes. Encoded by bacteriophage and phage-derived bacterial genes, Acrs prevent CRISPR-mediated inhibition of phage infection and can also block ...
Doudna, Jennifer A, Fellmann, Christof, Lew, Rachel J, McMahon, Blake, Shivram, Haridha, Watters, Kyle E   +5 more
core   +3 more sources

Research advances on pathogenic nucleic acid detection technology based on CRIPSR/Cas system

浙江大学学报. 农业与生命科学版, 2023
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) (CRISPR/Cas) system, an ancient bacterial and archaeal immune system, has rapidly developed into a popular gene-editing tool, which largely promotes the ...
LI Hongzhao, WANG Hao, YIN Rui, YUE Min, LI Yan   +4 more
doaj   +1 more source

Advances in Engineering the Fly Genome with the CRISPR-Cas System. [PDF]

, 2018
Drosophila has long been a premier model for the development and application of cutting-edge genetic approaches. The CRISPR-Cas system now adds the ability to manipulate the genome with ease and precision, providing a rich toolbox to interrogate ...
Bier, Ethan, Harrison, Melissa M, O'Connor-Giles, Kate M, Wildonger, Jill   +3 more
core   +2 more sources

CRISPR/Cas9‐mediated genome editing: from basic research to translational medicine [PDF]

, 2020
The recent development of the CRISPR/Cas9 system as an efficient and accessible programmable genome-editing tool has revolutionized basic science research. CRISPR/Cas9 system-based technologies have armed researchers with new powerful tools to unveil the
Ferreira, B I, Jacinto, Filipe, Link, Wolfgang   +2 more
core   +1 more source

CRISPR-Cas9/Cas12a biotechnology and application in bacteria

Synthetic and Systems Biotechnology, 2018
CRISPR-Cas technologies have greatly reshaped the biology field. In this review, we discuss the CRISPR-Cas with a particular focus on the associated technologies and applications of CRISPR-Cas9 and CRISPR-Cas12a, which have been most widely studied and ...
Ruilian Yao, Di Liu, Xiao Jia, Yuan Zheng, Wei Liu, Yi Xiao   +5 more
doaj   +1 more source

Associate toxin-antitoxin with CRISPR-Cas to kill multidrug-resistant pathogens

Nature Communications, 2023
CreTA, CRISPR-regulated toxin-antitoxin (TA), safeguards CRISPR-Cas immune systems by inducing cell dormancy/death upon their inactivation. Here, we characterize a bacterial CreTA associating with the I-F CRISPR-Cas in Acinetobacter.
Rui Wang, Xian Shu, Huiwei Zhao, Qiong Xue, Chao Liu, Aici Wu, Feiyue Cheng, Lingyun Wang, Yihan Zhang, Jie Feng, Nannan Wu, Ming Li   +11 more
doaj   +1 more source

Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana [PDF]

, 2016
Background: CRISPR-Cas is a recent and powerful addition to the molecular toolbox which allows programmable genome editing. It has been used to modify genes in a wide variety of organisms, but only two alga to date.
A Scheffel, A Xiao, Amanda Hopes, B Delalat, BJ Karas, C Bowler, C Brooks, C Fan, C Jeffryes, C Krysiak, CB Field, CUT Hellen, E Jabri, E Weber, E Weber, ES Lander, EV Armbrust, F Daboussi, FL Pinto, G d’Ippolito, H-L Xing, JA Doudna, JD Sander, JE Habel, JEN Dolatabadi, JG Doench, K Belhaj, M Jinek, M Nymark, N Poulsen, N Poulsen, NM Price, O Cook, P Kuczynska, P Stothard, PD Weyman, PG Falkowski, Q Zheng, R Tarleton, RJ Olson, RP Shrestha, S Gupta, S Malviya, S-E Shin, Sophien Kamoun, T Sakuma, TB Jacobs, Thomas Mock, TTY Doan, V Nekrasov, V Smetacek, Vladimir Nekrasov, W Li, WHCF Kooistra   +53 more
core   +2 more sources

Bacteriophage DNA glucosylation impairs target DNA binding by type I and II but not by type V CRISPR-Cas effector complexes [PDF]

, 2017
Prokaryotes encode various host defense systems that provide protection against mobile genetic elements. Restriction-modification (R-M) and CRISPR-Cas systems mediate host defense by sequence specific targeting of invasive DNA.
Anders, C., Brouns, S.J.J., Dickman, M.J., Houkes, J., Jinek, M., Kunne, T., Lochs, S.J.A., Mohanraju, P., Swarts, D.C., van der Oost, J., Vlot, M., Zheng, P.   +11 more
core   +6 more sources

Development and Application of CRISPR/Cas System in Rice

Rice Science, 2019
In the past several years, the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system has been harnessed as an efficient and powerful tool for targeted genome editing in different prokaryotic and ...
Ren Jun, Hu Xixun, Wang Kejian, Wang Chun   +3 more
doaj   +1 more source

Clustered Regularly Interspaced Short Palindromic Repeats-Associated Protein System for Resistance Against Plant Viruses: Applications and Perspectives

Frontiers in Plant Science, 2022
Different genome editing approaches have been used to engineer resistance against plant viruses. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas; CRISPR/Cas) systems to create pinpoint genetic ...
Fredy D. A. Silva, Elizabeth P. B. Fontes   +1 more
doaj   +1 more source